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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">hfb</journal-id><journal-title-group><journal-title xml:lang="ru">Health, Food &amp; Biotechnology</journal-title><trans-title-group xml:lang="en"><trans-title>Health, Food &amp; Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2712-7648</issn><publisher><publisher-name>РОСБИОТЕХ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.36107/hfb.2020.i1.s296</article-id><article-id custom-type="elpub" pub-id-type="custom">hfb-66</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОТЕХНОЛОГИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BIOTECHNOLOGY</subject></subj-group></article-categories><title-group><article-title>Усовершенствование технологии плодово-ягодных соков с использованием пектолитических ферментов</article-title><trans-title-group xml:lang="en"><trans-title>Improvement of Fruit-Berry Juices Technology using Pectolytic Enzymes</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бутова</surname><given-names>C. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Butova</surname><given-names>S. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бутова Светлана Николаевна</p></bio><bio xml:lang="en"><p>Svetlana N. Butova</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вольнова</surname><given-names>Е. Р.</given-names></name><name name-style="western" xml:lang="en"><surname>Volnova</surname><given-names>E. R.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Вольнова Екатерина Романовна</p></bio><bio xml:lang="en"><p>Ekaterina R. Volnova</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Николаева</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Nikolaeva</surname><given-names>Yu. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Николаева Юлия Владимировна</p><p>125080, город Москва, Волоколамское шоссе, дом 11</p></bio><bio xml:lang="en"><p>Yulia V. Nikolaeva</p><p>11 Volokolamskoe highway, Moscow, 125080</p></bio><email xlink:type="simple">organikamgupp@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Едличкова</surname><given-names>Я.</given-names></name><name name-style="western" xml:lang="en"><surname>Jedličková</surname><given-names>J.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Едличкова Яна</p><p>Прага</p></bio><bio xml:lang="en"><p>Jana Jedličková</p><p>Prague</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО «Московский государственный университет пищевых производств»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Moscow State University of Food Production</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ООО «AJETO»</institution><country>Чехия</country></aff><aff xml:lang="en"><institution>AJETO</institution><country>Czech Republic</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2020</year></pub-date><pub-date pub-type="epub"><day>08</day><month>05</month><year>2021</year></pub-date><volume>2</volume><issue>1</issue><fpage>128</fpage><lpage>139</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Бутова C.Н., Вольнова Е.Р., Николаева Ю.В., Едличкова Я., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Бутова C.Н., Вольнова Е.Р., Николаева Ю.В., Едличкова Я.</copyright-holder><copyright-holder xml:lang="en">Butova S.N., Volnova E.R., Nikolaeva Y.V., Jedličková J.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.hfb-journal.ru/jour/article/view/66">https://www.hfb-journal.ru/jour/article/view/66</self-uri><abstract><p>В условиях неблагоприятной экологической обстановки, нарушения структуры питания населения, ограниченности сырьевых растительных источников пищи существует потребность в высококачественных пищевых продуктах, отвечающих не только отечественным, но и зарубежным требованиям. Особое значение в структуре питания имеют плодово-ягодные соки и напитки на их основе, обладающие высокой пищевой ценностью. Перед соковой индустрией уже давно стоит вопрос создания технологии, позволяющей максимально извлекать сок без потери его питательных и органолептических свойств. В настоящей статье предложен способ усовершенствования технологии получения сока из яблок и черной смородины при помощи использования пектолитических ферментов, продуцентом которых являются дрожжи Zygofabospora marxiana BKM Y-848. В ходе проведения исследований было установлено, что действие дрожжевой эндополигалактуроназы (активность 3000 ед/см3) в дозировке от 0,004 до 0,01 % к массе сырья в диапазоне температур от 10 °С до 40 °С в течение 2-24 часов существенно увеличивает выход сока. При добавлении 0,01 % фермента к массе мезги при температуре 30 °С и продолжительности гидролиза 12 часов выход сока увеличился на 25-30 %. Также, в результате действия фермента снизилась кислотность соков на 15-45 % в зависимости от вида сырья. Количество сахаров в яблочном соке увеличилось на 3-5 %, в черносмородиновом соке содержание сахара возросло на 12-15 % по сравнению с соками, полученными из сырья, не подвергавшемуся ферментативной обработке. Ключевым моментов исследования стало снижение вязкости соков, что в дальнейшем существенно увеличило скорость фильтрации. Таким образом, была доказана целесообразность применения ферментов пектолитического действия в технологии соковых продуктов с целью её усовершенствования.</p></abstract><trans-abstract xml:lang="en"><p>In the conditions of unfavorable ecological situation, disruption of the nutrition structure of the population, limited raw plant food sources, there is a need for high-quality food products that meet not only domestic but also international requirements. Of particular importance in the nutrition structure are fruit and berry juices and drinks based on them, having high nutritional value. The juice industry has long been looking for a technology that allows extracting as much as possible juice without losing its nutritional and organoleptic properties. This article proposes a way to improve the technology of juice production from apples and blackcurrant using pectolytic enzymes obtained using Zygofabospora marxiana BKM Y-848 yeast. In the course of the studies it was found that yeast endopolygalacturonase (activity 3000 units/cm3) in a dosage from 0.004 to 0.01% of the weight of raw materials working within 2-24 hours at temperatures from 10 to 40 °C significantly increases the yield of juice. When adding 0.01% of the enzyme to the mass of skin and seeds’ pulp at a temperature of 30 °C and the duration of hydrolysis of 12 hours, the yield of juice increases by 25-30%. Also, the enzyme decreased acidity of juices by 15-45% depending on the type of raw material. The amount of sugars in apple juice increased by 3-5%, in blackcurrant juice the sugar content increased by 12-15% compared to juices obtained from raw materials without enzymatic processing. The key points of the study were the reduction of the juice viscosity, which further significantly increased the filtration rate. Thus, the advisability of usage of enzymes of pectolytic action in the technology of juice products aimed at its improvement it was proved.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>пектолитические ферменты</kwd><kwd>яблоки</kwd><kwd>черная смородина</kwd><kwd>сок</kwd><kwd>ферментативный гидролиз</kwd><kwd>дрожжи Zygofabospora marxiana BKM Y-848</kwd></kwd-group><kwd-group xml:lang="en"><kwd>pectolytic enzymes</kwd><kwd>apples</kwd><kwd>blackcurrant</kwd><kwd>juice</kwd><kwd>enzymatic hydrolysis</kwd><kwd>yeast Zygofabospora marxiana BKM Y-848</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Аверьянова, Е. В., &amp; Грищенко, О. В. (2015). Комплексная переработка вторичных продуктов сокового производства на примере жома жимолости. В Фундаментальные и прикладные аспекты биотехнологии (с. 98-100).</mixed-citation><mixed-citation xml:lang="en">Aver’yanova, E. 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