hfbHealth, Food & BiotechnologyHealth, Food & Biotechnology2712-7648РОСБИОТЕХ10.36107/hfb.2019.i4.s280hfb-54Research ArticleБИОТЕХНОЛОГИИBIOTECHNOLOGYПолучение клеточной культуры имбиряObtaining a Cell Culture of GingerИвановаЛ. А.IvanovaL. A.

Иванова Людмила Афанасьевна

125080, Москва, Волоколамское шоссе, дом 11

Lyudmila A. Ivanova

11 Volokolamskoe highway, Moscow, 125080, Russian Federation

biotech@mgupp.ruФоменкоИ. А.FomenkoI. A.

Фоменко Иван Андреевич

125080, Москва, Волоколамское шоссе, дом 11

127299, город Москва, ул. Клары Цеткин, дом 4

Ivan A. Fomenko

11 Volokolamskoe highway, Moscow, 125080, Russian Federation

4 Klara Cetkin str., Moscow, 127299, Russian Federation

iv.fomenko@mail.ruФоменкоВ. В.FomenkoV. V.

Фоменко Виктория Валерьевна

127299, город Москва, ул. Клары Цеткин, дом 4

Viktoriya V. Fomenko

Klara Cetkin str., Moscow, 127299, Russian Federation

victoria-96@bk.ruЧурмасоваЛ. А.ChurmasovaL. A.

Чурмасова Людмила Алексеевна

125080, Москва, Волоколамское шоссе, дом 11

Lyudmila A. Churmasova

11 Volokolamskoe highway, Moscow, 125080, Russian Federation

ludmila-churmasova@yandex.ruЩенниковаА. М.ShhennikovaA. M.

Щенникова Анастасия Михайловна

125080, Москва, Волоколамское шоссе, дом 11

Anastasiya M. Shhennikova

11 Volokolamskoe highway, Moscow, 125080, Russian Federation

nastya.shhennikova@inbox.ruФГБОУ ВО «Московский государственный университет пищевых производств»РоссияMoscow State University of Food ProductionRussian FederationФГБОУ ВО «Московский государственный университет пищевых производств»; Институт «Прикладной биохимии и машиностроения» (ОАО «Биохиммаш»)РоссияMoscow State University of Food Production; JSC “Biokhimmash”Russian FederationИнститут «Прикладной биохимии и машиностроения» (ОАО «Биохиммаш»)РоссияJSC “Biokhimmash”Russian Federation2019060520211492104Copyright © Иванова Л.А., Фоменко И.А., Фоменко В.В., Чурмасова Л.А., Щенникова А.М., 20202020Иванова Л.А., Фоменко И.А., Фоменко В.В., Чурмасова Л.А., Щенникова А.М.Ivanova L.A., Fomenko I.A., Fomenko V.V., Churmasova L.A., Shhennikova A.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.hfb-journal.ru/jour/article/view/54

Имбирь, как пряность и лекарственное растение с древних времен и по настоящее время пользуется большим спросом в Индии и в Китае благодаря широкому спектру действия на организм человека. В дикой природе сам имбирь не вырастает, поэтому в промышленных масштабах его выращивают на специальных плантациях, которые охватывают большие территории. Немаловажны для активного роста растения и климатические условия. Стандартные программы разведения имбиря затруднительны из-за низкой продуктивности и недостатка естественного количества семян. Биотехнологический метод культивирования клеток растений дает возможность получить неограниченное количество идентичных клеток за 3 - 4 недели. Исходным материалом для проведения исследований и получения каллусных культур клеток служили корневища, стебли и листья Zingiber sp. Для получения первичных каллусов и их дальнейшего культивирования использовали различные модификации среды Мурасиге и Скуга, а также среды Гамборга. Экспланты культивировали при температуре 26 °С и влажности 60–70 %, в темноте. Цикл выращивания составлял 25–30 суток. По результатам анализа были получены новые данные о свойствах культур клеток растений рода Zingiber, предложены пути решения ряда глобальных экологических проблем, а также в результате использования классического метода получения каллусной культуры стерильного интактного растения был получен новый исходный штамм клеток in vitro имбиря со стабильными ростовыми характеристиками.

Ginger, as a spice and medicinal plant from ancient times to the present, is in great demand in India and China due to the wide range of effects on the human body. In the wild, ginger does not grow by itself, therefore it is grown on special plantations covering large areas on an industrial scale. Climatic conditions are also important for active plant growth. Standard ginger breeding programs are difficult due to low productivity and lack of natural seed quantity. The biotechnological method of cultivating plant cells makes it possible to obtain an unlimited number of identical cells in 3 to 4 weeks. Zingiber sp. rhizomes, stems and leaves are served as the starting material for research and obtaining callus cell cultures. To obtain primary callus for their further cultivation, various modifications of the Murashige and Skoog medium, as well as the Hamburg medium, were used. Explants were cultured in the dark at 26 ° C and a humidity of 60–70% . The growing cycle was 25–30 days. According to the results of the analysis, new data on the properties of Zingiber plant cell cultures was obtained, ways to solve a number of global environmental problems were proposed. As a result of using the classical method of obtaining a callus culture of a sterile intact plant was obtained a new initial ginger cell strain in vitro with stable growth characteristics.

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The authors declare that there are no conflicts of interest present.