hfbHealth, Food & BiotechnologyHealth, Food & Biotechnology2712-7648РОСБИОТЕХhfb-298Research ArticleСтатьиМодификация хитозана и композиции на его основеhttps://orcid.org/0009-0008-9000-9274КунАлёна Дмитриевна

Кафедра «Промышленный дизайн, технология упаковки и экспертиза»,  аспирант

alyona.kun6@gmail.comhttps://orcid.org/0000-0003-2906-094XБезнаеваОльга Владимировна

Кафедра «Промышленный дизайн, технология упаковки и экспертиза»,  кандидат технических наук, доцент

olgabeznaeva@mgupp.ruhttps://orcid.org/0000-0003-3370-4226КиршИрина Анатольевна

Кафедра «Промышленный дизайн, технология упаковки и экспертиза»,  доктор химических наук, доцент

kirshia@mgupp.ruФГБОУ ВО «Российский биотехнологический университет (РОСБИОТЕХ)», г. Москва, РоссияRussian Federation20261310202683298Copyright © Кун А.Д., Безнаева О.В., Кирш И.А., 20262026Кун А.Д., Безнаева О.В., Кирш И.А.Кун А.Д., Безнаева О.В., Кирш И.А.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.hfb-journal.ru/jour/article/view/298

Существующая литература демонстрирует широкие возможности хитозана как биоразлагаемого полимера, однако разнообразие его свойств и методов модификации затрудняет формирование целостного представления о его функциональном потенциале. Настоящий обзор обобщает современные данные о получении хитина и хитозана из различных биологических источников, анализирует их физико-химические и механические характеристики, а также направления химической модификации, включая преобразования по аминогруппам, гидроксильным группам, сшивку и прививочную сополимеризацию. Показано, как эти модификации регулируют растворимость, биологическую активность и структурные свойства материала. Особое внимание уделено формированию композитных систем и влиянию нанонаполнителей и пластификаторов на прочность, эластичность и функциональность материалов. Обзор систематизирует биомедицинские, экологические и технологические применения модифицированного хитозана и демонстрирует его значимость как универсального биополимера нового поколения.

хитозанхитинбиополимербиоразлагаемый полимерхимическая модификацияN-модификацияO-модификациякватернизациясульфонированиефосфорилированиесшивкаграфтингацилированиетиолированиеоснования Шиффафизико-механические свойствабиологическая активностьантимикробная активностьбиосовместимостьрастворимостькомпозиты хитозанананонаполнителинаночастицынаноцеллюлозатканевая инженериядоставка лекарствраневые покрытияпищевая упаковкасорбентыэкологические применениязелёная химияReferencesAhmed, J., Mulla, M., Arfat, Y. A., & Thai T, L. A. (2017). Mechanical, thermal, structural and barrier properties of crab shell chitosan/graphene oxide composite films. Food Hydrocolloids, 71, 141–148. https://doi.org/10.1016/j.foodhyd.2017.05.013Ahmed, J., Mulla, M., Arfat, Y. A., & Thai T, L. A. (2017). Mechanical, thermal, structural and barrier properties of crab shell chitosan/graphene oxide composite films. Food Hydrocolloids, 71, 141–148. https://doi.org/10.1016/j.foodhyd.2017.05.013Alemu, D., Getachew, E., & Mondal, A. K. (2023). Study on the Physicochemical Properties of Chitosan and their Applications in the Biomedical Sector. International Journal of Polymer Science, 2023, 1–13. https://doi.org/10.1155/2023/5025341Alemu, D., Getachew, E., & Mondal, A. K. (2023). Study on the Physicochemical Properties of Chitosan and their Applications in the Biomedical Sector. International Journal of Polymer Science, 2023, 1–13. https://doi.org/10.1155/2023/5025341Ali, S. A., Ali, E. S., Hamdy, G., Badawy, M. S. E. M., Ismail, A. R., El-Sabbagh, I. A., El-Fass, M. M., & Elsawy, M. A. (2024). Enhancing physical characteristics and antibacterial efficacy of chitosan through investigation of microwave-assisted chemically formulated chitosan-coated ZnO and chitosan/ZnO physical composite. Scientific Reports, 14(1), 9348. https://doi.org/10.1038/s41598-024-58862-6Ali, S. A., Ali, E. S., Hamdy, G., Badawy, M. S. E. M., Ismail, A. R., El-Sabbagh, I. A., El-Fass, M. M., & Elsawy, M. A. (2024). Enhancing physical characteristics and antibacterial efficacy of chitosan through investigation of microwave-assisted chemically formulated chitosan-coated ZnO and chitosan/ZnO physical composite. Scientific Reports, 14(1), 9348. https://doi.org/10.1038/s41598-024-58862-6Al-Naamani, L., Dobretsov, S., & Dutta, J. (2016). Chitosan-zinc oxide nanoparticle composite coating for active food packaging applications. Innovative Food Science & Emerging Technologies, 38, 231–237. https://doi.org/10.1016/j.ifset.2016.10.010Al-Naamani, L., Dobretsov, S., & Dutta, J. (2016). Chitosan-zinc oxide nanoparticle composite coating for active food packaging applications. Innovative Food Science & Emerging Technologies, 38, 231–237. https://doi.org/10.1016/j.ifset.2016.10.010Al-qarhami, F., Abdallah, A. B., Khalifa, M. E., & Awad, F. S. (2025). Glutaraldehyde-crosslinked magnetic chitosan nanocomposite for efficient Cr(VI) removal: A sustainable approach to aquatic remediation. International Journal of Biological Macromolecules, 310, 143459. https://doi.org/10.1016/j.ijbiomac.2025.143459Al-qarhami, F., Abdallah, A. B., Khalifa, M. E., & Awad, F. S. (2025). Glutaraldehyde-crosslinked magnetic chitosan nanocomposite for efficient Cr(VI) removal: A sustainable approach to aquatic remediation. International Journal of Biological Macromolecules, 310, 143459. https://doi.org/10.1016/j.ijbiomac.2025.143459Alven, S., Aderibigbe, B. A., Alven, S., & Aderibigbe, B. A. (2024). Chitosan-Based Scaffolds Incorporated with Silver Nanoparticles for the Treatment of Infected Wounds. Pharmaceutics, 16(3). https://doi.org/10.3390/pharmaceutics16030327Alven, S., Aderibigbe, B. A., Alven, S., & Aderibigbe, B. A. (2024). Chitosan-Based Scaffolds Incorporated with Silver Nanoparticles for the Treatment of Infected Wounds. Pharmaceutics, 16(3). https://doi.org/10.3390/pharmaceutics16030327Anastas, P. T., & Warner, J. C. (2000). Green Chemistry: Theory and Practice. Oxford University PressOxford. https://doi.org/10.1093/oso/9780198506980.001.0001Anastas, P. T., & Warner, J. C. (2000). Green Chemistry: Theory and Practice. Oxford University PressOxford. https://doi.org/10.1093/oso/9780198506980.001.0001Aranaz, I., Alcántara, A. R., Civera, M. C., Arias, C., Elorza, B., Heras Caballero, A., & Acosta, N. (2021). Chitosan: An Overview of Its Properties and Applications. Polymers, 13(19), 3256. https://doi.org/10.3390/polym13193256Aranaz, I., Alcántara, A. R., Civera, M. C., Arias, C., Elorza, B., Heras Caballero, A., & Acosta, N. (2021). Chitosan: An Overview of Its Properties and Applications. Polymers, 13(19), 3256. https://doi.org/10.3390/polym13193256Barbosa, R. F. S., Shyam, S., Misra, S., Mitra, S. K., & Rosa, D. S. (2025). Chitosan Hydrogels Crosslinked with Glutaraldehyde for Potential Toxic Elements Removal: Batch and Purification Device Analysis. Journal of Applied Polymer Science, 142(39), e57539. https://doi.org/10.1002/app.57539Barbosa, R. F. S., Shyam, S., Misra, S., Mitra, S. K., & Rosa, D. S. (2025). Chitosan Hydrogels Crosslinked with Glutaraldehyde for Potential Toxic Elements Removal: Batch and Purification Device Analysis. Journal of Applied Polymer Science, 142(39), e57539. https://doi.org/10.1002/app.57539Barjasteh, M., Dehnavi, S. M., Ahmadi Seyedkhani, S., Rahnamaee, S. Y., & Golizadeh, M. (2023). Improved biological activities of dual nanofibrous chitosan/bacterial cellulose wound dressing by a novel silver-based metal-organic framework. Surfaces and Interfaces, 36, 102631. https://doi.org/10.1016/j.surfin.2023.102631Barjasteh, M., Dehnavi, S. M., Ahmadi Seyedkhani, S., Rahnamaee, S. Y., & Golizadeh, M. (2023). Improved biological activities of dual nanofibrous chitosan/bacterial cellulose wound dressing by a novel silver-based metal-organic framework. Surfaces and Interfaces, 36, 102631. https://doi.org/10.1016/j.surfin.2023.102631Berezin, B. B., Kulikov, S. N., Zhuravleva, I. L., Ezernitskaya, M. G., Piskarev, V. E., Antonov, Y. A., Bezrodnykh, E. A., & Tikhonov, V. E. (2025). Complexation with chicken yolk phosvitin modulates antibacterial activity of chitosan. Carbohydrate Polymers, 362, 123691. https://doi.org/10.1016/j.carbpol.2025.123691Berezin, B. B., Kulikov, S. N., Zhuravleva, I. L., Ezernitskaya, M. G., Piskarev, V. E., Antonov, Y. A., Bezrodnykh, E. A., & Tikhonov, V. E. (2025). Complexation with chicken yolk phosvitin modulates antibacterial activity of chitosan. Carbohydrate Polymers, 362, 123691. https://doi.org/10.1016/j.carbpol.2025.123691Bhardwaj, N., & Kundu, S. C. (2010). Electrospinning: A fascinating fiber fabrication technique. Biotechnology Advances, 28(3), 325–347. https://doi.org/10.1016/j.biotechadv.2010.01.004Bhardwaj, N., & Kundu, S. C. (2010). Electrospinning: A fascinating fiber fabrication technique. Biotechnology Advances, 28(3), 325–347. https://doi.org/10.1016/j.biotechadv.2010.01.004Bhowmik, S., Agyei, D., & Ali, A. (2024). Biodegradable chitosan hydrogel film incorporated with polyvinyl alcohol, chitooligosaccharides, and gallic acid for potential application in food packaging. Cellulose, 31(13), 8087–8103. https://doi.org/10.1007/s10570-024-06080-8Bhowmik, S., Agyei, D., & Ali, A. (2024). Biodegradable chitosan hydrogel film incorporated with polyvinyl alcohol, chitooligosaccharides, and gallic acid for potential application in food packaging. Cellulose, 31(13), 8087–8103. https://doi.org/10.1007/s10570-024-06080-8Binder, L., de Sousa Santos, F., & da Conceição, T. F. (2025). Sustainable smart coating of chitosan, halloysite nanotubes and phenolic acids for corrosion protection of Al alloy. Materials Chemistry and Physics, 345, 131240. https://doi.org/10.1016/j.matchemphys.2025.131240Binder, L., de Sousa Santos, F., & da Conceição, T. F. (2025). Sustainable smart coating of chitosan, halloysite nanotubes and phenolic acids for corrosion protection of Al alloy. Materials Chemistry and Physics, 345, 131240. https://doi.org/10.1016/j.matchemphys.2025.131240Bof, M. J., Bordagaray, V. C., Locaso, D. E., & García, M. A. (2015). Chitosan molecular weight effect on starch-composite film properties. Food Hydrocolloids, 51, 281–294. https://doi.org/10.1016/j.foodhyd.2015.05.018Bof, M. J., Bordagaray, V. C., Locaso, D. E., & García, M. A. (2015). Chitosan molecular weight effect on starch-composite film properties. Food Hydrocolloids, 51, 281–294. https://doi.org/10.1016/j.foodhyd.2015.05.018Borrelle, S. B., Ringma, J. (2020). Predicted growth in plastic waste exceeds efforts to mitigate plastic pollution. Science. https://doi.org/10.1126/science.aba3656Borrelle, S. B., Ringma, J. (2020). Predicted growth in plastic waste exceeds efforts to mitigate plastic pollution. Science. https://doi.org/10.1126/science.aba3656Brizga, J., Hubacek, K., & Feng, K. (2020). The Unintended Side Effects of Bioplastics: Carbon, Land, and Water Footprints. One Earth, 3(1), 45–53. https://doi.org/10.1016/j.oneear.2020.06.016Brizga, J., Hubacek, K., & Feng, K. (2020). The Unintended Side Effects of Bioplastics: Carbon, Land, and Water Footprints. One Earth, 3(1), 45–53. https://doi.org/10.1016/j.oneear.2020.06.016Carvalho, L. C. R., Queda, F., Santos, C. V. A., & Marques, M. M. B. (2016). Selective Modification of Chitin and Chitosan: En Route to Tailored Oligosaccharides. Chemistry – An Asian Journal, 11(24), 3468–3481. https://doi.org/10.1002/asia.201601041Carvalho, L. C. R., Queda, F., Santos, C. V. A., & Marques, M. M. B. (2016). Selective Modification of Chitin and Chitosan: En Route to Tailored Oligosaccharides. Chemistry – An Asian Journal, 11(24), 3468–3481. https://doi.org/10.1002/asia.201601041Cazón, P., & Vázquez, M. (2020). Mechanical and barrier properties of chitosan combined with other components as food packaging film. Environmental Chemistry Letters, 18(2), 257–267. https://doi.org/10.1007/s10311-019-00936-3Cazón, P., & Vázquez, M. (2020). Mechanical and barrier properties of chitosan combined with other components as food packaging film. Environmental Chemistry Letters, 18(2), 257–267. https://doi.org/10.1007/s10311-019-00936-3Chacon, E. L., Bertolo, M. R. V. (2023). Collagen-chitosan-hydroxyapatite composite scaffolds for bone repair in ovariectomized rats. Scientific Reports, 13(1), 28. https://doi.org/10.1038/s41598-022-24424-xChacon, E. L., Bertolo, M. R. V. (2023). Collagen-chitosan-hydroxyapatite composite scaffolds for bone repair in ovariectomized rats. Scientific Reports, 13(1), 28. https://doi.org/10.1038/s41598-022-24424-xChen, Q., Qi, Y., Jiang, Y., Quan, W., Luo, H., Wu, K., Li, S., & Ouyang, Q. (2022). Progress in Research of Chitosan Chemical Modification Technologies and Their Applications. Marine Drugs, 20(8), 536. https://doi.org/10.3390/md20080536Chen, Q., Qi, Y., Jiang, Y., Quan, W., Luo, H., Wu, K., Li, S., & Ouyang, Q. (2022). Progress in Research of Chitosan Chemical Modification Technologies and Their Applications. Marine Drugs, 20(8), 536. https://doi.org/10.3390/md20080536Chicea, D., & Nicolae-Maranciuc, A. (2024). A Review of Chitosan-Based Materials for Biomedical, Food, and Water Treatment Applications. Materials, 17(23), 5770. https://doi.org/10.3390/ma17235770Chicea, D., & Nicolae-Maranciuc, A. (2024). A Review of Chitosan-Based Materials for Biomedical, Food, and Water Treatment Applications. Materials, 17(23), 5770. https://doi.org/10.3390/ma17235770Da Silva, A. B., Rufato, K. B., De Oliveira. (2020). Composite materials based on chitosan/gold nanoparticles: From synthesis to biomedical applications. International Journal of Biological Macromolecules, 161, 977–998. https://doi.org/10.1016/j.ijbiomac.2020.06.113Da Silva, A. B., Rufato, K. B., De Oliveira. (2020). Composite materials based on chitosan/gold nanoparticles: From synthesis to biomedical applications. International Journal of Biological Macromolecules, 161, 977–998. https://doi.org/10.1016/j.ijbiomac.2020.06.113Dash, M., Chiellini, F., Ottenbrite, R. M., & Chiellini, E. (2011). Chitosan—A versatile semi-synthetic polymer in biomedical applications. Progress in Polymer Science, 36(8), 981–1014. https://doi.org/10.1016/j.progpolymsci.2011.02.001Dash, M., Chiellini, F., Ottenbrite, R. M., & Chiellini, E. (2011). Chitosan—A versatile semi-synthetic polymer in biomedical applications. Progress in Polymer Science, 36(8), 981–1014. https://doi.org/10.1016/j.progpolymsci.2011.02.001De Silva, R. T., Mantilaka, M. M. M. G. P. G., Ratnayake, S. P., Amaratunga, G. A. J., & De Silva, K. M. N. (2017). Nano-MgO reinforced chitosan nanocomposites for high performance packaging applications with improved mechanical, thermal and barrier properties. Carbohydrate Polymers, 157, 739–747. https://doi.org/10.1016/j.carbpol.2016.10.038De Silva, R. T., Mantilaka, M. M. M. G. P. G., Ratnayake, S. P., Amaratunga, G. A. J., & De Silva, K. M. N. (2017). Nano-MgO reinforced chitosan nanocomposites for high performance packaging applications with improved mechanical, thermal and barrier properties. Carbohydrate Polymers, 157, 739–747. https://doi.org/10.1016/j.carbpol.2016.10.038DeVierno Kreuder, A., House-Knight, T. (2017). A Method for Assessing Greener Alternatives between Chemical Products Following the 12 Principles of Green Chemistry. ACS Sustainable Chemistry & Engineering, 5(4), 2927–2935. https://doi.org/10.1021/acssuschemeng.6b02399DeVierno Kreuder, A., House-Knight, T. (2017). A Method for Assessing Greener Alternatives between Chemical Products Following the 12 Principles of Green Chemistry. ACS Sustainable Chemistry & Engineering, 5(4), 2927–2935. https://doi.org/10.1021/acssuschemeng.6b02399Dong, H., Feng, C., Zhu, J., Gu, X. (2024). Ultrasmall gold Nanoparticles/Carboxymethyl chitosan composite hydrogel: Tough, restorable, biocompatible antimicrobial dressing for wound healing. Applied Materials Today, 38, 102206. https://doi.org/10.1016/j.apmt.2024.102206Dong, H., Feng, C., Zhu, J., Gu, X. (2024). Ultrasmall gold Nanoparticles/Carboxymethyl chitosan composite hydrogel: Tough, restorable, biocompatible antimicrobial dressing for wound healing. Applied Materials Today, 38, 102206. https://doi.org/10.1016/j.apmt.2024.102206Du, Y., Shi, B., Luan, X., Wang, Y., & Song, H. (2023). Chitosan/cellulose nanocrystal biocomposite coating for fruit postharvest preservation. Industrial Crops and Products, 205, 117543. https://doi.org/10.1016/j.indcrop.2023.117543Du, Y., Shi, B., Luan, X., Wang, Y., & Song, H. (2023). Chitosan/cellulose nanocrystal biocomposite coating for fruit postharvest preservation. Industrial Crops and Products, 205, 117543. https://doi.org/10.1016/j.indcrop.2023.117543Elmehbad, N. Y., Mohamed, N. A., Abd El-Ghany, N. A., & Abdel-Aziz, M. M. (2023). Reinforcement of the antimicrobial activity and biofilm inhibition of novel chitosan-based hydrogels utilizing zinc oxide nanoparticles. International Journal of Biological Macromolecules, 246, 125582. https://doi.org/10.1016/j.ijbiomac.2023.125582Elmehbad, N. Y., Mohamed, N. A., Abd El-Ghany, N. A., & Abdel-Aziz, M. M. (2023). Reinforcement of the antimicrobial activity and biofilm inhibition of novel chitosan-based hydrogels utilizing zinc oxide nanoparticles. International Journal of Biological Macromolecules, 246, 125582. https://doi.org/10.1016/j.ijbiomac.2023.125582El-Meligy, M. A., El-Monaem, E. M. A., Eltaweil, A. S. (2024). Recent Advancements in Metallic Au- and Ag-Based Chitosan Nanocomposite Derivatives for Enhanced Anticancer Drug Delivery. Molecules, 29(10). https://doi.org/10.3390/molecules29102393El-Meligy, M. A., El-Monaem, E. M. A., Eltaweil, A. S. (2024). Recent Advancements in Metallic Au- and Ag-Based Chitosan Nanocomposite Derivatives for Enhanced Anticancer Drug Delivery. Molecules, 29(10). https://doi.org/10.3390/molecules29102393Elsabee, M. Z., Morsi, R. E., & Fathy, M. (2020). Chemical Modifications of Chitin and Chitosan. В S. Kim (Ред.), Encyclopedia of Marine Biotechnology (1-е изд., сс. 885–963). Wiley. https://doi.org/10.1002/9781119143802.ch36Elsabee, M. Z., Morsi, R. E., & Fathy, M. (2020). Chemical Modifications of Chitin and Chitosan. В S. Kim (Ред.), Encyclopedia of Marine Biotechnology (1-е изд., сс. 885–963). Wiley. https://doi.org/10.1002/9781119143802.ch36Feng, Q., Fan, B., He, Y.-C., & Ma, C. (2024). Antibacterial, antioxidant and fruit packaging ability of biochar-based silver nanoparticles-polyvinyl alcohol-chitosan composite film. International Journal of Biological Macromolecules, 256, 128297. https://doi.org/10.1016/j.ijbiomac.2023.128297Feng, Q., Fan, B., He, Y.-C., & Ma, C. (2024). Antibacterial, antioxidant and fruit packaging ability of biochar-based silver nanoparticles-polyvinyl alcohol-chitosan composite film. International Journal of Biological Macromolecules, 256, 128297. https://doi.org/10.1016/j.ijbiomac.2023.128297Francis, A. O., Zaini, M. A. A., Muhammad, I. M., Abdulsalam, S., & El-Nafaty, U. A. (2023). Physicochemical modification of chitosan adsorbent: A perspective. Biomass Conversion and Biorefinery, 13(7), 5557–5575. https://doi.org/10.1007/s13399-021-01599-3Francis, A. O., Zaini, M. A. A., Muhammad, I. M., Abdulsalam, S., & El-Nafaty, U. A. (2023). Physicochemical modification of chitosan adsorbent: A perspective. Biomass Conversion and Biorefinery, 13(7), 5557–5575. https://doi.org/10.1007/s13399-021-01599-3Geng, Y., Xue, H., Zhang, Z., Panayi, A. C., Knoedler, S., Zhou, W., Mi, B., & Liu, G. (2023). Recent advances in carboxymethyl chitosan-based materials for biomedical applications. Carbohydrate Polymers, 305, 120555. https://doi.org/10.1016/j.carbpol.2023.120555Geng, Y., Xue, H., Zhang, Z., Panayi, A. C., Knoedler, S., Zhou, W., Mi, B., & Liu, G. (2023). Recent advances in carboxymethyl chitosan-based materials for biomedical applications. Carbohydrate Polymers, 305, 120555. https://doi.org/10.1016/j.carbpol.2023.120555Getahun, M. J., Kassie, B. B., & Alemu, T. S. (2024). Recent advances in biopolymer synthesis, properties, & commercial applications: A review. Process Biochemistry, 145, 261–287. https://doi.org/10.1016/j.procbio.2024.06.034Getahun, M. J., Kassie, B. B., & Alemu, T. S. (2024). Recent advances in biopolymer synthesis, properties, & commercial applications: A review. Process Biochemistry, 145, 261–287. https://doi.org/10.1016/j.procbio.2024.06.034Han, D., Yan, L., Chen, W., & Li, W. (2011). Preparation of chitosan/graphene oxide composite film with enhanced mechanical strength in the wet state. Carbohydrate Polymers, 83(2), 653–658. https://doi.org/10.1016/j.carbpol.2010.08.038Han, D., Yan, L., Chen, W., & Li, W. (2011). Preparation of chitosan/graphene oxide composite film with enhanced mechanical strength in the wet state. Carbohydrate Polymers, 83(2), 653–658. https://doi.org/10.1016/j.carbpol.2010.08.038Herliana, H., Yusuf, H. Y., Laviana, A., Wandawa, G. (2023). Characterization and Analysis of Chitosan-Gelatin Composite-Based Biomaterial Effectivity as Local Hemostatic Agent: A Systematic Review. Polymers, 15(3). https://doi.org/10.3390/polym15030575Herliana, H., Yusuf, H. Y., Laviana, A., Wandawa, G. (2023). Characterization and Analysis of Chitosan-Gelatin Composite-Based Biomaterial Effectivity as Local Hemostatic Agent: A Systematic Review. Polymers, 15(3). https://doi.org/10.3390/polym15030575Hu, D., Ren, Q., Li, Z., & Zhang, L. (2020). Chitosan-Based Biomimetically Mineralized Composite Materials in Human Hard Tissue Repair. Molecules, 25(20), 4785. https://doi.org/10.3390/molecules25204785Hu, D., Ren, Q., Li, Z., & Zhang, L. (2020). Chitosan-Based Biomimetically Mineralized Composite Materials in Human Hard Tissue Repair. Molecules, 25(20), 4785. https://doi.org/10.3390/molecules25204785Huq, T., Khan, A., Brown, D., Dhayagude, N., He, Z., & Ni, Y. (2022). Sources, production and commercial applications of fungal chitosan: A review. Journal of Bioresources and Bioproducts, 7(2), 85–98. https://doi.org/10.1016/j.jobab.2022.01.002Huq, T., Khan, A., Brown, D., Dhayagude, N., He, Z., & Ni, Y. (2022). Sources, production and commercial applications of fungal chitosan: A review. Journal of Bioresources and Bioproducts, 7(2), 85–98. https://doi.org/10.1016/j.jobab.2022.01.002Jadhav, S., Khivansara, R., & Sherje, A. P. (2025). Chitosan-pectin complexes: A new frontier in biopolymer-based drug delivery systems. Journal of Biomaterials Science, Polymer Edition, 0(0), 1–32. https://doi.org/10.1080/09205063.2025.2561007Jadhav, S., Khivansara, R., & Sherje, A. P. (2025). Chitosan-pectin complexes: A new frontier in biopolymer-based drug delivery systems. Journal of Biomaterials Science, Polymer Edition, 0(0), 1–32. https://doi.org/10.1080/09205063.2025.2561007Jha, S., Akula, B., Enyioma, H., Novak, M., Amin, V., & Liang, H. (2024). Biodegradable Biobased Polymers: A Review of the State of the Art, Challenges, and Future Directions. Polymers, 16(16), 2262. https://doi.org/10.3390/polym16162262Jha, S., Akula, B., Enyioma, H., Novak, M., Amin, V., & Liang, H. (2024). Biodegradable Biobased Polymers: A Review of the State of the Art, Challenges, and Future Directions. Polymers, 16(16), 2262. https://doi.org/10.3390/polym16162262Joseph, S. R., Danti, S., Sebastian, L., V.S, N., A., S. C., & M., U. (2025). Sustainable innovations: Chitosan-cellulose nanocrystal composites for enhanced mechanical, antibacterial, and photocatalytic applications. Biomass Conversion and Biorefinery, 15(7), 10855–10871. https://doi.org/10.1007/s13399-024-05971-xJoseph, S. R., Danti, S., Sebastian, L., V.S, N., A., S. C., & M., U. (2025). Sustainable innovations: Chitosan-cellulose nanocrystal composites for enhanced mechanical, antibacterial, and photocatalytic applications. Biomass Conversion and Biorefinery, 15(7), 10855–10871. https://doi.org/10.1007/s13399-024-05971-xKasi, P. B., Azar, M. G., Dodda, J. M., Bělský, P., Kovářík, T., Šlouf, M., Dobrá, J. K., & Babuška, V. (2023). Chitosan and cellulose-based composite hydrogels with embedded titanium dioxide nanoparticles as candidates for biomedical applications. International Journal of Biological Macromolecules, 243, 125334. https://doi.org/10.1016/j.ijbiomac.2023.125334Kasi, P. B., Azar, M. G., Dodda, J. M., Bělský, P., Kovářík, T., Šlouf, M., Dobrá, J. K., & Babuška, V. (2023). Chitosan and cellulose-based composite hydrogels with embedded titanium dioxide nanoparticles as candidates for biomedical applications. International Journal of Biological Macromolecules, 243, 125334. https://doi.org/10.1016/j.ijbiomac.2023.125334Khayrova, A., Lopatin, S., & Varlamov, V. (2021). Obtaining chitin, chitosan and their melanin complexes from insects. International Journal of Biological Macromolecules, 167, 1319–1328. https://doi.org/10.1016/j.ijbiomac.2020.11.086Khayrova, A., Lopatin, S., & Varlamov, V. (2021). Obtaining chitin, chitosan and their melanin complexes from insects. International Journal of Biological Macromolecules, 167, 1319–1328. https://doi.org/10.1016/j.ijbiomac.2020.11.086Kumar, J. A., Sathish, S., Prabu, D., Giri, J., Makki, E., Jayaprabakar, J., Ziyayeva, G. K., Baigenzhenov, O., Sathish, T., & Praveenkumar, T. R. (2024). Waste shrimp shell mediated Chitosan-Magnesium Oxide nanocomposite: Synthesis, characterization and exploitation towards acenaphthene removal from aqueous solution. Alexandria Engineering Journal, 104, 124–135. https://doi.org/10.1016/j.aej.2024.06.014Kumar, J. A., Sathish, S., Prabu, D., Giri, J., Makki, E., Jayaprabakar, J., Ziyayeva, G. K., Baigenzhenov, O., Sathish, T., & Praveenkumar, T. R. (2024). Waste shrimp shell mediated Chitosan-Magnesium Oxide nanocomposite: Synthesis, characterization and exploitation towards acenaphthene removal from aqueous solution. Alexandria Engineering Journal, 104, 124–135. https://doi.org/10.1016/j.aej.2024.06.014Kumar, S., & Maiti, P. (2016). Controlled biodegradation of polymers using nanoparticles and its application. RSC Advances, 6(72), 67449–67480. https://doi.org/10.1039/C6RA08641AKumar, S., & Maiti, P. (2016). Controlled biodegradation of polymers using nanoparticles and its application. RSC Advances, 6(72), 67449–67480. https://doi.org/10.1039/C6RA08641AKurul, F., Doruk, B., & Topkaya, S. N. (2025). Principles of green chemistry: Building a sustainable future. Discover Chemistry, 2(1), 68. https://doi.org/10.1007/s44371-025-00152-9Kurul, F., Doruk, B., & Topkaya, S. N. (2025). Principles of green chemistry: Building a sustainable future. Discover Chemistry, 2(1), 68. https://doi.org/10.1007/s44371-025-00152-9Kyzas, G., & Bikiaris, D. (2015). Recent Modifications of Chitosan for Adsorption Applications: A Critical and Systematic Review. Marine Drugs, 13(1), 312–337. https://doi.org/10.3390/md13010312Kyzas, G., & Bikiaris, D. (2015). Recent Modifications of Chitosan for Adsorption Applications: A Critical and Systematic Review. Marine Drugs, 13(1), 312–337. https://doi.org/10.3390/md13010312Leceta, I., Guerrero, P., & De La Caba, K. (2013). Functional properties of chitosan-based films. Carbohydrate Polymers, 93(1), 339–346. https://doi.org/10.1016/j.carbpol.2012.04.031Leceta, I., Guerrero, P., & De La Caba, K. (2013). Functional properties of chitosan-based films. Carbohydrate Polymers, 93(1), 339–346. https://doi.org/10.1016/j.carbpol.2012.04.031Lewandowska, K. (2015). Characterization of chitosan composites with synthetic polymers and inorganic additives. International Journal of Biological Macromolecules, 81, 159–164. https://doi.org/10.1016/j.ijbiomac.2015.08.003Lewandowska, K. (2015). Characterization of chitosan composites with synthetic polymers and inorganic additives. International Journal of Biological Macromolecules, 81, 159–164. https://doi.org/10.1016/j.ijbiomac.2015.08.003Lin, C., Lu, T.-W., Hsu, F.-Y., Huang, T.-W., Ho, M.-H., Lu, H.-T., & Mi, F.-L. (2025). An injectable in situ-forming hydrogel with self-activating genipin-chitosan (GpCS) cross-linking and an O2/Ca2+ self-supplying capability for wound healing and rapid hemostasis. Carbohydrate Polymers, 351, 123051. https://doi.org/10.1016/j.carbpol.2024.123051Lin, C., Lu, T.-W., Hsu, F.-Y., Huang, T.-W., Ho, M.-H., Lu, H.-T., & Mi, F.-L. (2025). An injectable in situ-forming hydrogel with self-activating genipin-chitosan (GpCS) cross-linking and an O2/Ca2+ self-supplying capability for wound healing and rapid hemostasis. Carbohydrate Polymers, 351, 123051. https://doi.org/10.1016/j.carbpol.2024.123051Liu, F., Zhang, X., Xiao, X., Duan, Q., Bai, H., Cao, Y., Zhang, Y., Alee, M., & Yu, L. (2023). Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging. Carbohydrate Polymers, 312, 120755. https://doi.org/10.1016/j.carbpol.2023.120755Liu, F., Zhang, X., Xiao, X., Duan, Q., Bai, H., Cao, Y., Zhang, Y., Alee, M., & Yu, L. (2023). Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging. Carbohydrate Polymers, 312, 120755. https://doi.org/10.1016/j.carbpol.2023.120755Liu, S., Zhao, Y., Xu, M., Wen, J., Wang, H., Yan, H., Gao, X., Niu, B., & Li, W. (2024). Antibacterial photodynamic properties of silver nanoparticles-loaded curcumin composite material in chitosan-based films. International Journal of Biological Macromolecules, 256, 128014. https://doi.org/10.1016/j.ijbiomac.2023.128014Liu, S., Zhao, Y., Xu, M., Wen, J., Wang, H., Yan, H., Gao, X., Niu, B., & Li, W. (2024). Antibacterial photodynamic properties of silver nanoparticles-loaded curcumin composite material in chitosan-based films. International Journal of Biological Macromolecules, 256, 128014. https://doi.org/10.1016/j.ijbiomac.2023.128014Liu, X., Xu, Y., Liao, W., Guo, C., Gan, M., & Wang, Q. (2023). Preparation and characterization of chitosan/bacterial cellulose composite biodegradable films combined with curcumin and its application on preservation of strawberries. Food Packaging and Shelf Life, 35, 101006. https://doi.org/10.1016/j.fpsl.2022.101006Liu, X., Xu, Y., Liao, W., Guo, C., Gan, M., & Wang, Q. (2023). Preparation and characterization of chitosan/bacterial cellulose composite biodegradable films combined with curcumin and its application on preservation of strawberries. Food Packaging and Shelf Life, 35, 101006. https://doi.org/10.1016/j.fpsl.2022.101006Liu, X., Zhao, X., Liu, Y., & Zhang, T. (2022). Review on preparation and adsorption properties of chitosan and chitosan composites. Polymer Bulletin, 79(4), 2633–2665. https://doi.org/10.1007/s00289-021-03626-9Liu, X., Zhao, X., Liu, Y., & Zhang, T. (2022). Review on preparation and adsorption properties of chitosan and chitosan composites. Polymer Bulletin, 79(4), 2633–2665. https://doi.org/10.1007/s00289-021-03626-9Madera-Santana, T. J., Herrera-Méndez, C. H., & Rodríguez-Núñez, J. R. (2018). An overview of the chemical modifications of chitosan and their advantages. Green Materials, 6(4), 131–142. https://doi.org/10.1680/jgrma.18.00053Madera-Santana, T. J., Herrera-Méndez, C. H., & Rodríguez-Núñez, J. R. (2018). An overview of the chemical modifications of chitosan and their advantages. Green Materials, 6(4), 131–142. https://doi.org/10.1680/jgrma.18.00053Muthu, M., Gopal, J., Chun, S., Devadoss, A. J. P., Hasan, N., & Sivanesan, I. (2021). Crustacean Waste-Derived Chitosan: Antioxidant Properties and Future Perspective. Antioxidants, 10(2), 228. https://doi.org/10.3390/antiox10020228Muthu, M., Gopal, J., Chun, S., Devadoss, A. J. P., Hasan, N., & Sivanesan, I. (2021). Crustacean Waste-Derived Chitosan: Antioxidant Properties and Future Perspective. Antioxidants, 10(2), 228. https://doi.org/10.3390/antiox10020228Navas, A. F. N., Araujo-Rodríguez, D. G., Valencia-Llano, C.-H., Insuasty, D. (2024). Lyophilized Polyvinyl Alcohol and Chitosan Scaffolds Pre-Loaded with Silicon Dioxide Nanoparticles for Tissue Regeneration. Molecules, 29(16). https://doi.org/10.3390/molecules29163850Navas, A. F. N., Araujo-Rodríguez, D. G., Valencia-Llano, C.-H., Insuasty, D. (2024). Lyophilized Polyvinyl Alcohol and Chitosan Scaffolds Pre-Loaded with Silicon Dioxide Nanoparticles for Tissue Regeneration. Molecules, 29(16). https://doi.org/10.3390/molecules29163850Negm, N. A., Hefni, H. H. H., Abd-Elaal, A. A. A., Badr, E. A., & Abou Kana, M. T. H. (2020). Advancement on modification of chitosan biopolymer and its potential applications. International Journal of Biological Macromolecules, 152, 681–702. https://doi.org/10.1016/j.ijbiomac.2020.02.196Negm, N. A., Hefni, H. H. H., Abd-Elaal, A. A. A., Badr, E. A., & Abou Kana, M. T. H. (2020). Advancement on modification of chitosan biopolymer and its potential applications. International Journal of Biological Macromolecules, 152, 681–702. https://doi.org/10.1016/j.ijbiomac.2020.02.196Ozcan, A., Arman Kandirmaz, E., & Acar Buyukpehlivan, G. (2023). Chitosan-titanium nanoparticle coated papers for active packaging. Journal of Food Engineering, 356, 111584. https://doi.org/10.1016/j.jfoodeng.2023.111584Ozcan, A., Arman Kandirmaz, E., & Acar Buyukpehlivan, G. (2023). Chitosan-titanium nanoparticle coated papers for active packaging. Journal of Food Engineering, 356, 111584. https://doi.org/10.1016/j.jfoodeng.2023.111584Pal, K., Bharti, D., Sarkar, P., Anis, A., Kim, D., Chałas, R., Maksymiuk, P., Stachurski, P., & Jarzębski, M. (2021). Selected Applications of Chitosan Composites. International Journal of Molecular Sciences, 22(20), 10968. https://doi.org/10.3390/ijms222010968Pal, K., Bharti, D., Sarkar, P., Anis, A., Kim, D., Chałas, R., Maksymiuk, P., Stachurski, P., & Jarzębski, M. (2021). Selected Applications of Chitosan Composites. International Journal of Molecular Sciences, 22(20), 10968. https://doi.org/10.3390/ijms222010968Pandya, T., Joshi, D., Presswala, Z., Kulkarni, M., Patel, R., Patel, S., Bhattacharya, S., & Prajapati, B. G. (2025). Advanced therapeutic strategies using Thermo-sensitive chitosan/pectin hydrogel in the treatment of multiple cancers. Carbohydrate Polymers, 357, 123454. https://doi.org/10.1016/j.carbpol.2025.123454Pandya, T., Joshi, D., Presswala, Z., Kulkarni, M., Patel, R., Patel, S., Bhattacharya, S., & Prajapati, B. G. (2025). Advanced therapeutic strategies using Thermo-sensitive chitosan/pectin hydrogel in the treatment of multiple cancers. Carbohydrate Polymers, 357, 123454. https://doi.org/10.1016/j.carbpol.2025.123454Petrova, V. A., Dubashynskaya, N. V., Zhuravskii, S. G. (2025). Design, Characterization, and Enhanced Performance of Electrospun Chitosan-Based Nanocomposites Reinforced with Halloysite Nanotubes and Cerium Oxide Nanoparticles for Wound Healing Applications. International Journal of Molecular Sciences, 26(21). https://doi.org/10.3390/ijms262110520Petrova, V. A., Dubashynskaya, N. V., Zhuravskii, S. G. (2025). Design, Characterization, and Enhanced Performance of Electrospun Chitosan-Based Nanocomposites Reinforced with Halloysite Nanotubes and Cerium Oxide Nanoparticles for Wound Healing Applications. International Journal of Molecular Sciences, 26(21). https://doi.org/10.3390/ijms262110520Piekarska, K., Sikora, M., Owczarek, M., Jóźwik-Pruska, J., & Wiśniewska-Wrona, M. (2023). Chitin and Chitosan as Polymers of the Future—Obtaining, Modification, Life Cycle Assessment and Main Directions of Application. Polymers, 15(4), 793. https://doi.org/10.3390/polym15040793Piekarska, K., Sikora, M., Owczarek, M., Jóźwik-Pruska, J., & Wiśniewska-Wrona, M. (2023). Chitin and Chitosan as Polymers of the Future—Obtaining, Modification, Life Cycle Assessment and Main Directions of Application. Polymers, 15(4), 793. https://doi.org/10.3390/polym15040793Pokhrel, S., & Yadav, P. N. (2019). Functionalization of chitosan polymer and their applications. Journal of Macromolecular Science, Part A, 56(5), 450–475. https://doi.org/10.1080/10601325.2019.1581576Pokhrel, S., & Yadav, P. N. (2019). Functionalization of chitosan polymer and their applications. Journal of Macromolecular Science, Part A, 56(5), 450–475. https://doi.org/10.1080/10601325.2019.1581576Saberian, M., Roudsari, R. S., Haghshenas, N., Rousta, A., & Alizadeh, S. (2024). How the combination of alginate and chitosan can fabricate a hydrogel with favorable properties for wound healing. Heliyon, 10(11). https://doi.org/10.1016/j.heliyon.2024.e32040Saberian, M., Roudsari, R. S., Haghshenas, N., Rousta, A., & Alizadeh, S. (2024). How the combination of alginate and chitosan can fabricate a hydrogel with favorable properties for wound healing. Heliyon, 10(11). https://doi.org/10.1016/j.heliyon.2024.e32040Sahariah, P., & Másson, M. (2017). Antimicrobial Chitosan and Chitosan Derivatives: A Review of the Structure–Activity Relationship. Biomacromolecules, 18(11), 3846–3868. https://doi.org/10.1021/acs.biomac.7b01058Sahariah, P., & Másson, M. (2017). Antimicrobial Chitosan and Chitosan Derivatives: A Review of the Structure–Activity Relationship. Biomacromolecules, 18(11), 3846–3868. https://doi.org/10.1021/acs.biomac.7b01058Saka, C. (2024). Surface modification strategies for nanoparticles based on chitosan and titanium dioxide: Catalytic properties and mechanism for sodium borohydride dehydrogenation in methanol. Fuel, 356, 129552. https://doi.org/10.1016/j.fuel.2023.129552Saka, C. (2024). Surface modification strategies for nanoparticles based on chitosan and titanium dioxide: Catalytic properties and mechanism for sodium borohydride dehydrogenation in methanol. Fuel, 356, 129552. https://doi.org/10.1016/j.fuel.2023.129552Samokhin, Y., Varava, Y., Diedkova, K., Yanko, I. (2023). Fabrication and Characterization of Electrospun Chitosan/Polylactic Acid (CH/PLA) Nanofiber Scaffolds for Biomedical Application. Journal of Functional Biomaterials, 14(8). https://doi.org/10.3390/jfb14080414Samokhin, Y., Varava, Y., Diedkova, K., Yanko, I. (2023). Fabrication and Characterization of Electrospun Chitosan/Polylactic Acid (CH/PLA) Nanofiber Scaffolds for Biomedical Application. Journal of Functional Biomaterials, 14(8). https://doi.org/10.3390/jfb14080414Sazanova, T. S., Otvagina, K. V., & Vorotyntsev, I. V. (2018). The contributions of supramolecular organization to mechanical properties of chitosan and chitosan copolymers with synthetic polymers according to atomic force microscopy. Polymer Testing, 68, 350–358. https://doi.org/10.1016/j.polymertesting.2018.04.031Sazanova, T. S., Otvagina, K. V., & Vorotyntsev, I. V. (2018). The contributions of supramolecular organization to mechanical properties of chitosan and chitosan copolymers with synthetic polymers according to atomic force microscopy. Polymer Testing, 68, 350–358. https://doi.org/10.1016/j.polymertesting.2018.04.031Semenyuk, V. S., & Nikitin, E. A. (2021). System Development for Liquid Chemicals Point Injection Based on Convolutional Neural Network Models. Agricultural Machinery and Technologies, 15(2), 41–45. https://doi.org/10.22314/2073-7599-2021-15-1-41-45Semenyuk, V. S., & Nikitin, E. A. (2021). System Development for Liquid Chemicals Point Injection Based on Convolutional Neural Network Models. Agricultural Machinery and Technologies, 15(2), 41–45. https://doi.org/10.22314/2073-7599-2021-15-1-41-45Sen, T., Ozcelik, B., Qiao, G. G., & Ozmen, M. M. (2017). Hierarchical porous hybrid chitosan scaffolds with tailorable mechanical properties. Materials Letters, 209, 528–531. https://doi.org/10.1016/j.matlet.2017.08.088Sen, T., Ozcelik, B., Qiao, G. G., & Ozmen, M. M. (2017). Hierarchical porous hybrid chitosan scaffolds with tailorable mechanical properties. Materials Letters, 209, 528–531. https://doi.org/10.1016/j.matlet.2017.08.088Shrestha, R., Thenissery, A., Khupse, R., & Rajashekara, G. (2023). Strategies for the Preparation of Chitosan Derivatives for Antimicrobial, Drug Delivery, and Agricultural Applications: A Review. Molecules, 28(22), 7659. https://doi.org/10.3390/molecules28227659Shrestha, R., Thenissery, A., Khupse, R., & Rajashekara, G. (2023). Strategies for the Preparation of Chitosan Derivatives for Antimicrobial, Drug Delivery, and Agricultural Applications: A Review. Molecules, 28(22), 7659. https://doi.org/10.3390/molecules28227659Si, J., Yang, Y., Xing, X., Yang, F., & Shan, P. (2019). Controlled degradable chitosan/collagen composite scaffolds for application in nerve tissue regeneration. Polymer Degradation and Stability, 166, 73–85. https://doi.org/10.1016/j.polymdegradstab.2019.05.023Si, J., Yang, Y., Xing, X., Yang, F., & Shan, P. (2019). Controlled degradable chitosan/collagen composite scaffolds for application in nerve tissue regeneration. Polymer Degradation and Stability, 166, 73–85. https://doi.org/10.1016/j.polymdegradstab.2019.05.023Song, L., Chen, Y., Liu, Z., Zhu, L., Song, L., Zhang, M., Xue, T., Lv, B., Liu, H., & Zhang, X. (2023). Preparation, characterization, and stability assessment of a nano-delivery system loaded with phosvitin phosphopeptide-calcium chelate. Food Bioscience, 56, 103306. https://doi.org/10.1016/j.fbio.2023.103306Song, L., Chen, Y., Liu, Z., Zhu, L., Song, L., Zhang, M., Xue, T., Lv, B., Liu, H., & Zhang, X. (2023). Preparation, characterization, and stability assessment of a nano-delivery system loaded with phosvitin phosphopeptide-calcium chelate. Food Bioscience, 56, 103306. https://doi.org/10.1016/j.fbio.2023.103306Souza, V. G. L., Pires, J. R. A., Rodrigues, C., Coelhoso, I. M., & Fernando, A. L. (2020). Chitosan Composites in Packaging Industry—Current Trends and Future Challenges. Polymers, 12(2), 417. https://doi.org/10.3390/polym12020417Souza, V. G. L., Pires, J. R. A., Rodrigues, C., Coelhoso, I. M., & Fernando, A. L. (2020). Chitosan Composites in Packaging Industry—Current Trends and Future Challenges. Polymers, 12(2), 417. https://doi.org/10.3390/polym12020417State of art review on sustainable biodegradable polymers with a market overview for sustainability packaging. (2024). Materials Today Sustainability, 26, 100776. https://doi.org/10.1016/j.mtsust.2024.100776State of art review on sustainable biodegradable polymers with a market overview for sustainability packaging. (2024). Materials Today Sustainability, 26, 100776. https://doi.org/10.1016/j.mtsust.2024.100776Tagandurdyyeva, N. A., Trube, M. A., Shemyakin, I. O., Solomitskiy, D. N. (2023). Properties of Resorbable Conduits Based on Poly(L-Lactide) Nanofibers and Chitosan Fibers for Peripheral Nerve Regeneration. Polymers, 15(15). https://doi.org/10.3390/polym15153323Tagandurdyyeva, N. A., Trube, M. A., Shemyakin, I. O., Solomitskiy, D. N. (2023). Properties of Resorbable Conduits Based on Poly(L-Lactide) Nanofibers and Chitosan Fibers for Peripheral Nerve Regeneration. Polymers, 15(15). https://doi.org/10.3390/polym15153323Talón, E., Trifkovic, K. T., Nedovic, V. A., Bugarski, B. M., Vargas, M., Chiralt, A., & González-Martínez, C. (2017). Antioxidant edible films based on chitosan and starch containing polyphenols from thyme extracts. Carbohydrate Polymers, 157, 1153–1161. https://doi.org/10.1016/j.carbpol.2016.10.080Talón, E., Trifkovic, K. T., Nedovic, V. A., Bugarski, B. M., Vargas, M., Chiralt, A., & González-Martínez, C. (2017). Antioxidant edible films based on chitosan and starch containing polyphenols from thyme extracts. Carbohydrate Polymers, 157, 1153–1161. https://doi.org/10.1016/j.carbpol.2016.10.080Tasselli, F., Mirmohseni, A., Seyed Dorraji, M. S., & Figoli, A. (2013). Mechanical, swelling and adsorptive properties of dry–wet spun chitosan hollow fibers crosslinked with glutaraldehyde. Reactive and Functional Polymers, 73(1), 218–223. https://doi.org/10.1016/j.reactfunctpolym.2012.08.007Tasselli, F., Mirmohseni, A., Seyed Dorraji, M. S., & Figoli, A. (2013). Mechanical, swelling and adsorptive properties of dry–wet spun chitosan hollow fibers crosslinked with glutaraldehyde. Reactive and Functional Polymers, 73(1), 218–223. https://doi.org/10.1016/j.reactfunctpolym.2012.08.007Thambiliyagodage, C., Jayanetti, M., Mendis, A., Ekanayake, G., Liyanaarachchi, H., & Vigneswaran, S. (2023). Recent Advances in Chitosan-Based Applications—A Review. Materials, 16(5), 2073. https://doi.org/10.3390/ma16052073Thambiliyagodage, C., Jayanetti, M., Mendis, A., Ekanayake, G., Liyanaarachchi, H., & Vigneswaran, S. (2023). Recent Advances in Chitosan-Based Applications—A Review. Materials, 16(5), 2073. https://doi.org/10.3390/ma16052073Universiti Tun Hussein Onn Malaysia, Abd Rahman, S. N. F., Othman, S. A., & Universiti Tun Hussein Onn Malaysia. (2024). Chitosan Properties: A Preliminary Review. Journal of Advanced Mechanical Engineering Applications, 5(1). https://doi.org/10.30880/jamea.2024.05.01.002Universiti Tun Hussein Onn Malaysia, Abd Rahman, S. N. F., Othman, S. A., & Universiti Tun Hussein Onn Malaysia. (2024). Chitosan Properties: A Preliminary Review. Journal of Advanced Mechanical Engineering Applications, 5(1). https://doi.org/10.30880/jamea.2024.05.01.002Verardi, A., Sangiorgio, P., Moliterni, S., Errico, S., Spagnoletta, A., & Dimatteo, S. (2023). Advanced technologies for chitin recovery from crustacean waste. Clean Technologies and Recycling, 3(1), 4–43. https://doi.org/10.3934/ctr.2023002Verardi, A., Sangiorgio, P., Moliterni, S., Errico, S., Spagnoletta, A., & Dimatteo, S. (2023). Advanced technologies for chitin recovery from crustacean waste. Clean Technologies and Recycling, 3(1), 4–43. https://doi.org/10.3934/ctr.2023002Wahba, M. I. (2020). Enhancement of the mechanical properties of chitosan. Journal of Biomaterials Science, Polymer Edition, 31(3), 350–375. https://doi.org/10.1080/09205063.2019.1692641Wahba, M. I. (2020). Enhancement of the mechanical properties of chitosan. Journal of Biomaterials Science, Polymer Edition, 31(3), 350–375. https://doi.org/10.1080/09205063.2019.1692641Wahba, M. I. (2024). A comprehensive review on genipin: An efficient natural cross-linker for biopolymers. Polymer Bulletin, 81(16), 14251–14305. https://doi.org/10.1007/s00289-024-05406-7Wahba, M. I. (2024). A comprehensive review on genipin: An efficient natural cross-linker for biopolymers. Polymer Bulletin, 81(16), 14251–14305. https://doi.org/10.1007/s00289-024-05406-7Wang, K., Wu, L., Li, Y., & Li, H. (2023). Preparation and characterization of chitosan/halloysite nanotubes composite film with ethylene scavenging and gas resistance for active food packaging. Journal of Food Safety, 43(2), e13027. https://doi.org/10.1111/jfs.13027Wang, K., Wu, L., Li, Y., & Li, H. (2023). Preparation and characterization of chitosan/halloysite nanotubes composite film with ethylene scavenging and gas resistance for active food packaging. Journal of Food Safety, 43(2), e13027. https://doi.org/10.1111/jfs.13027Wang, W., Meng, Q., Li, Q., Liu, J., Zhou, M., Jin, Z., & Zhao, K. (2020). Chitosan Derivatives and Their Application in Biomedicine. International Journal of Molecular Sciences, 21(2), 487. https://doi.org/10.3390/ijms21020487Wang, W., Meng, Q., Li, Q., Liu, J., Zhou, M., Jin, Z., & Zhao, K. (2020). Chitosan Derivatives and Their Application in Biomedicine. International Journal of Molecular Sciences, 21(2), 487. https://doi.org/10.3390/ijms21020487Wang, Z., Qu, J., Zhong, H., Mukerabigwi, J. F., Zeng, J., Wang, Y., & Cao, Y. (2025). An Enhanced Undergraduate Experiment on Chitosan Purification and Hydrogel Preparation Using a Safer Cross-Linking Approach. Journal of Chemical Education, 102(8), 3528–3535. https://doi.org/10.1021/acs.jchemed.5c00283Wang, Z., Qu, J., Zhong, H., Mukerabigwi, J. F., Zeng, J., Wang, Y., & Cao, Y. (2025). An Enhanced Undergraduate Experiment on Chitosan Purification and Hydrogel Preparation Using a Safer Cross-Linking Approach. Journal of Chemical Education, 102(8), 3528–3535. https://doi.org/10.1021/acs.jchemed.5c00283Wawrzyńczak, A., Chudzińska, J., & Feliczak‐Guzik, A. (2024). Metal and Metal Oxides Nanoparticles as Nanofillers for Biodegradable Polymers. ChemPhysChem, 25(10), e202300823. https://doi.org/10.1002/cphc.202300823Wawrzyńczak, A., Chudzińska, J., & Feliczak‐Guzik, A. (2024). Metal and Metal Oxides Nanoparticles as Nanofillers for Biodegradable Polymers. ChemPhysChem, 25(10), e202300823. https://doi.org/10.1002/cphc.202300823Wen, H., Nan, S., Wu, D., Sun, Q., Tong, Y., Zhang, J., Jin, S., & Shen, W. (2023). A Systematic Review on Intensifications of Artificial Intelligence Assisted Green Solvent Development. Industrial & Engineering Chemistry Research, 62(48), 20473–20491. https://doi.org/10.1021/acs.iecr.3c02305Wen, H., Nan, S., Wu, D., Sun, Q., Tong, Y., Zhang, J., Jin, S., & Shen, W. (2023). A Systematic Review on Intensifications of Artificial Intelligence Assisted Green Solvent Development. Industrial & Engineering Chemistry Research, 62(48), 20473–20491. https://doi.org/10.1021/acs.iecr.3c02305Wrońska, N., Katir, N., Nowak-Lange, M. (2023). Biodegradable Chitosan-Based Films as an Alternative to Plastic Packaging. Foods, 12(18). https://doi.org/10.3390/foods12183519Wrońska, N., Katir, N., Nowak-Lange, M. (2023). Biodegradable Chitosan-Based Films as an Alternative to Plastic Packaging. Foods, 12(18). https://doi.org/10.3390/foods12183519Wu, T., Farnood, R., O’Kelly, K., & Chen, B. (2014). Mechanical behavior of transparent nanofibrillar cellulose–chitosan nanocomposite films in dry and wet conditions. Journal of the Mechanical Behavior of Biomedical Materials, 32, 279–286. https://doi.org/10.1016/j.jmbbm.2014.01.014Wu, T., Farnood, R., O’Kelly, K., & Chen, B. (2014). Mechanical behavior of transparent nanofibrillar cellulose–chitosan nanocomposite films in dry and wet conditions. Journal of the Mechanical Behavior of Biomedical Materials, 32, 279–286. https://doi.org/10.1016/j.jmbbm.2014.01.014Xie, H., Chen, X., Shen, X., He, Y., Chen, W. (2018). Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing. International Journal of Biological Macromolecules, 107, 93–104. https://doi.org/10.1016/j.ijbiomac.2017.08.142Xie, H., Chen, X., Shen, X., He, Y., Chen, W. (2018). Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing. International Journal of Biological Macromolecules, 107, 93–104. https://doi.org/10.1016/j.ijbiomac.2017.08.142Xie, W., Li, T., Tiraferri, A., Drioli, E., Figoli, A., Crittenden, J. C., & Liu, B. (2021). Toward the Next Generation of Sustainable Membranes from Green Chemistry Principles. ACS Sustainable Chemistry & Engineering, 9(1), 50–75. https://doi.org/10.1021/acssuschemeng.0c07119Xie, W., Li, T., Tiraferri, A., Drioli, E., Figoli, A., Crittenden, J. C., & Liu, B. (2021). Toward the Next Generation of Sustainable Membranes from Green Chemistry Principles. ACS Sustainable Chemistry & Engineering, 9(1), 50–75. https://doi.org/10.1021/acssuschemeng.0c07119Xu, Y. X., Kim, K. M., Hanna, M. A., & Nag, D. (2005). Chitosan–starch composite film: Preparation and characterization. Industrial Crops and Products, 21(2), 185–192. https://doi.org/10.1016/j.indcrop.2004.03.002Xu, Y. X., Kim, K. M., Hanna, M. A., & Nag, D. (2005). Chitosan–starch composite film: Preparation and characterization. Industrial Crops and Products, 21(2), 185–192. https://doi.org/10.1016/j.indcrop.2004.03.002Xue, C., & Wilson, L. D. (2021). An Overview of the Design of Chitosan-Based Fiber Composite Materials. Journal of Composites Science, 5(6), 160. https://doi.org/10.3390/jcs5060160Xue, C., & Wilson, L. D. (2021). An Overview of the Design of Chitosan-Based Fiber Composite Materials. Journal of Composites Science, 5(6), 160. https://doi.org/10.3390/jcs5060160Yadav, M., Maurya, A. K., Behera, K., Chiu, F.-C., & Rhee, K. Y. (2024). Physical properties of cellulose nanocrystal/magnesium oxide/chitosan transparent composite films for packaging applications. International Journal of Biological Macromolecules, 264, 130560. https://doi.org/10.1016/j.ijbiomac.2024.130560Yadav, M., Maurya, A. K., Behera, K., Chiu, F.-C., & Rhee, K. Y. (2024). Physical properties of cellulose nanocrystal/magnesium oxide/chitosan transparent composite films for packaging applications. International Journal of Biological Macromolecules, 264, 130560. https://doi.org/10.1016/j.ijbiomac.2024.130560Yu, B. H., van de Pas, R., & Zahidi, I. (2025). Polylactic Acid (PLA), Polycaprolactone (PCL), and Chitosan: The Biodegradable Transition in Medical Waste Management. Advanced Sustainable Systems, 9(12), e00636. https://doi.org/10.1002/adsu.202500636Yu, B. H., van de Pas, R., & Zahidi, I. (2025). Polylactic Acid (PLA), Polycaprolactone (PCL), and Chitosan: The Biodegradable Transition in Medical Waste Management. Advanced Sustainable Systems, 9(12), e00636. https://doi.org/10.1002/adsu.202500636Zeng, X., Sun, Z., Chen, L., Zhang, X., Guo, X., & Li, G. (2025). Co-assembled biomimetic fibrils from collagen and chitosan for performance-enhancing hemostatic dressing. Biomaterials Science, 13(1), 236–249. https://doi.org/10.1039/D4BM01211AZeng, X., Sun, Z., Chen, L., Zhang, X., Guo, X., & Li, G. (2025). Co-assembled biomimetic fibrils from collagen and chitosan for performance-enhancing hemostatic dressing. Biomaterials Science, 13(1), 236–249. https://doi.org/10.1039/D4BM01211AZhang, K., Liu, Y., Shi, X., Zhang, R., He, Y., Zhang, H., & Wang, W. (2023). Application of polyvinyl alcohol/chitosan copolymer hydrogels in biomedicine: A review. International Journal of Biological Macromolecules, 242, 125192. https://doi.org/10.1016/j.ijbiomac.2023.125192Zhang, K., Liu, Y., Shi, X., Zhang, R., He, Y., Zhang, H., & Wang, W. (2023). Application of polyvinyl alcohol/chitosan copolymer hydrogels in biomedicine: A review. International Journal of Biological Macromolecules, 242, 125192. https://doi.org/10.1016/j.ijbiomac.2023.125192Zhu, Y., Zhang, Y., & Zhou, Y. (2022). Application Progress of Modified Chitosan and Its Composite Biomaterials for Bone Tissue Engineering. International Journal of Molecular Sciences, 23(12), 6574. https://doi.org/10.3390/ijms23126574Zhu, Y., Zhang, Y., & Zhou, Y. (2022). Application Progress of Modified Chitosan and Its Composite Biomaterials for Bone Tissue Engineering. International Journal of Molecular Sciences, 23(12), 6574. https://doi.org/10.3390/ijms23126574Zigrayová, D., Mikušová, V., & Mikuš, P. (2024). Advances in Chitosan Derivatives: Preparation, Properties and Applications in Pharmacy and Medicine. Gels, 10(11), 701. https://doi.org/10.3390/gels10110701Zigrayová, D., Mikušová, V., & Mikuš, P. (2024). Advances in Chitosan Derivatives: Preparation, Properties and Applications in Pharmacy and Medicine. Gels, 10(11), 701. https://doi.org/10.3390/gels10110701

The authors declare that there are no conflicts of interest present.