<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">hfb</journal-id><journal-title-group><journal-title xml:lang="ru">Health, Food &amp; Biotechnology</journal-title><trans-title-group xml:lang="en"><trans-title>Health, Food &amp; Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2712-7648</issn><publisher><publisher-name>РОСБИОТЕХ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.36107/hfb.2019.i3.s267</article-id><article-id custom-type="elpub" pub-id-type="custom">hfb-26</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОТЕХНОЛОГИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BIOTECHNOLOGY</subject></subj-group></article-categories><title-group><article-title>Микробиологическая безопасность меда</article-title><trans-title-group xml:lang="en"><trans-title>Microbiological Safety of Honey</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кущ</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kushch</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кущ Ирина Вячеславовна</p><p>125080, Москва, Волоколамское шоссе, дом 11</p></bio><bio xml:lang="en"><p>Irina V. Kushch</p><p>11 Volokolamskoe highway, Moscow, 125080 </p></bio><email xlink:type="simple">i.kusch@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ваннер</surname><given-names>Н. Э.</given-names></name><name name-style="western" xml:lang="en"><surname>Vanner</surname><given-names>N. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ваннер Наталья Эдуардовна</p><p>123022, город Москва, Звенигородское ш., дом 5</p></bio><bio xml:lang="en"><p>Natalya E. Vanner</p><p>5 Zvenigorodskoe highway, Moscow, 123022 </p></bio><email xlink:type="simple">vanner.natalia@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Удавлиев</surname><given-names>Д. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Udavliev</surname><given-names>D. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Удавлиев Дамир Исмаилович</p><p>125080, Москва, Волоколамское шоссе, дом 11</p></bio><bio xml:lang="en"><p>Damir I. Udavliev</p><p>11 Volokolamskoe highway, Moscow, 125080 </p></bio><email xlink:type="simple">udavlievdi@mgupp.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мурадова</surname><given-names>М. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Muradova</surname><given-names>M. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мурадова Мая Дурдымурадовна</p><p>744000, город Ашхабад, ул. Гёроглы, дом 62</p></bio><bio xml:lang="en"><p>Maya D. Muradova</p><p>62 Gorogly str., Ashgabat, 744000 </p></bio><email xlink:type="simple">mouradovamd@gmail.com</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО «Московский государственный университет пищевых производств»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Moscow State University of Food Production</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Всероссийский научно-исследовательский институт ветеринарной санитарии, гигиены и экологии — филиал ФГБНУ ФНЦ ВИЭВ РАН</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology - Branch of Federal State Budget Scientific Institution «Federal Scientific Center - K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences»</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Туркменский сельскохозяйственный университет имени С. А. Ниязова</institution><country>Туркменистан</country></aff><aff xml:lang="en"><institution>Turkmen Agricultural University Named after S.A.Niyazov</institution><country>Turkmenistan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>04</day><month>05</month><year>2021</year></pub-date><volume>1</volume><issue>3</issue><fpage>106</fpage><lpage>117</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кущ И.В., Ваннер Н.Э., Удавлиев Д.И., Мурадова М.Д., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Кущ И.В., Ваннер Н.Э., Удавлиев Д.И., Мурадова М.Д.</copyright-holder><copyright-holder xml:lang="en">Kushch I.V., Vanner N.E., Udavliev D.I., Muradova M.D.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.hfb-journal.ru/jour/article/view/26">https://www.hfb-journal.ru/jour/article/view/26</self-uri><abstract><p>Проведено микробиологическое исследование пчелиного меда реализуемого на рынках Москвы в отношении патогенных и условно-патогенных микроорганизмов (бактерии рода Salmonella, Shigella, общая микробная обсемененность (КМАФАнМ), бактерии группы кишечной палочки (БГКП), плесневых грибов, осмофильных дрожжей, S. aureus). Дополнительно были проведены органолептические (внешний вид, аромат, вкус) и физико-химические (определение массовой доли сахарозы, фруктозы и глюкозы, гидрокси-метилфурфураля, кислотности, электропроводность и т.д.) исследования для исключения фальсификации продукта. Микробиологические, органолептические и физико-химические исследования проводились в испытательной лаборатории соответствующей требованиям ГОСТ ISO/EC 17025-2019. Исследованию были представлены 221 образец меда, отобранные с 13 рынков Москвы на наличие патогенных и условно-патогенных микроорганизмов. По результатам исследований в 37 образцах (16,7 %) были обнаружены Escherichia coli, в 18 образцах (8 %) - бактерии группы кишечной палочки (БГКП), 53 образцах (23,9 %) - S.aureus, КМАФАнМ - 25 (11,3 %), осмофильные дрожжи - 3 (1,3 %), несоответствие по физико-химическим показателям обнаружено в 45 (20,3 %) образцах. Рассмотрены российские и международные требования к микробиологической безопасности меда в отношении патогенных и условно-патогенных микроорганизмов. Проведен анализ и сравнительная характеристика показателей и их значений.</p></abstract><trans-abstract xml:lang="en"><p>A microbiological study of bee honey sold in the Moscow markets with respect to pathogenic and conditionally pathogenic microorganisms (bacteria of the genus Salmonella, Shigella, total microbial contamination (KMAFAnM), bacteria of the E. coli group, mold, osmophilic yeast, S. aureus) was carried out. Additionally, organoleptic (appearance, aroma, taste) and physicochemical (determination of the mass fraction of sucrose, fructose and glucose, hydroxymethylfurfural, acidity, electrical conductivity, etc.) were conducted to exclude product falsification. Microbiological, organoleptic and physico-chemical studies were carried out in a testing laboratory that meets the requirements of GOST ISO / EC 17025-2019. The study presented 221 honey samples taken from 13 Moscow markets for the presence of pathogenic and conditionally pathogenic microorganisms. According to the results of studies, Escherichia coli was found in 37 samples (16.7 %), E. coli bacteria (BHEC) in 18 samples (8 %), S.aureus, 25 KMAFAnM (53 samples (23.9 %)) 11.3 %), osmophilic yeast - 3 (1.3 %), a discrepancy in physical and chemical parameters was found in 45 (20.3 %) samples. Considered Russian and international requirements for the microbiological safety of honey in relation to pathogenic and conditionally pathogenic microorganisms. Analyzed the comparative characteristics of indicators and their values.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>мед</kwd><kwd>микробиологическая безопасность</kwd><kwd>международные требования</kwd><kwd>патогенные</kwd><kwd>условно-патогенные микроорганизмы</kwd></kwd-group><kwd-group xml:lang="en"><kwd>honey</kwd><kwd>microbiological safety</kwd><kwd>international requirements</kwd><kwd>pathogenic and conditionally pathogenic microorganisms</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Бутко, М. П., Герасимов, А. С., Посконная, Т. Ф., Смирнов, А. М., Клочко, Р. Т., &amp; Попов, П.А. (2019). Требования по обеспечению безопасности и ветеринарно-санитарная экспертиза мёда пчелиного. Издательский дом «Научная библиотека».</mixed-citation><mixed-citation xml:lang="en">Butko, M. P., Gerasimov, A. S., Poskonnaya, T. F., Smirnov, A. M., Klochko, R. T., &amp; Popov, P. A. (2019). Trebovaniya po obespecheniyu bezopasnosti i veter-inarno-sanitarnoy ekspertizy moda pchelinogo [Requirements for ensuring safety and veterinary sanitary examination of bee honey]. Scientific Library  Publishing House</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Жунева, Л. С., Семченко, М. В., &amp; Асякина, Л. К. (2019). Анализ технологий получения сухого меда. Хранение и переработка сельхозсырья, 2, 8-23. https://doi.org/10.36107/spfp.2019.69</mixed-citation><mixed-citation xml:lang="en">Zhuneva, L. S., Semchenko, M. V., &amp; Asyakina, L. K. (2019). Analysis of technologies for obtaining dry honey. Khraneniye i pererabotka sel’khozsyr’ya [Storage and processing of agricultural raw materials], 2, 8-23. https://doi.org/10.36107/spfp.2019.69</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Салимов, Р. М. (2008). Выживаемость микроорганизмов в некоторых пищевых продуктах. Дальневосточный аграрный вестник, 4 (8), 25-27.</mixed-citation><mixed-citation xml:lang="en">Salimov, R. M. (2008). Microorganism survival in certain foods. Dal’nevostochnyy agrarnyy vestnik [Far Eastern Agricultural Bulletin], 4(8), 25-27. https://doi.org/10.24411/1999-6837-2008-00074</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Al-Nahari, A. A. M., Almasaudi, S. B., Abd El-Ghany, E. S. M., Barbour, E., Al Jaouni, S. K., &amp; Harakeh, S.(2015). Antimicrobial activities of Saudi honey against Pseudomonas aeruginosa. Saudi Journal of Biological Sciences, 22(5), 521-525. https://doi.org/10.1016/j.sjbs.2015.04.006</mixed-citation><mixed-citation xml:lang="en">Al-Nahari, A. A. M., Almasaudi, S. B., Abd El-Ghany, E. S. M., Barbour, E., Al Jaouni, S. K., &amp; Harakeh, S.(2015). Antimicrobial activities of Saudi honey against Pseudomonas aeruginosa. Saudi Journal of Biological Sciences, 22(5), 521-525. https://doi.org/10.1016/j.sjbs.2015.04.006</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Al-Waili, N., Salom, K., Al-Ghamdi, A., &amp; Ansari, M. J. (2012). Antibiotic, pesticide, and microbial contaminants of honey: human health hazards. The Scientific World Journal, 2012, 930849, https://doi.org/10.1100/2012/930849</mixed-citation><mixed-citation xml:lang="en">Al-Waili, N., Salom, K., Al-Ghamdi, A., &amp; Ansari, M. J. (2012). Antibiotic, pesticide, and microbial contaminants of honey: human health hazards. The Scientific World Journal, 2012, 930849, https://doi.org/10.1100/2012/930849</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Anand, S., Deighton, M., Livanos, G., Morrison, P.D., Pang, E. C. K., &amp; Mantri, N. (2019) Antimicrobial activity of agastache honey and characterization of its bioactive compounds in comparison with important commercial honeys. Frontiers in Microbiology, 10(263), 1-16. https://doi.org/10.3389/fmicb.2019.00263</mixed-citation><mixed-citation xml:lang="en">Anand, S., Deighton, M., Livanos, G., Morrison, P.D., Pang, E. C. K., &amp; Mantri, N. (2019) Antimicrobial activity of agastache honey and characterization of its bioactive compounds in comparison with important commercial honeys. Frontiers in Microbiology, 10(263), 1-16. https://doi.org/10.3389/fmicb.2019.00263</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Augustin, J.-C., &amp; Carlier, V. (2006). Lessons from the organization of a proficiency testing program in food microbiology by interlaboratory comparison: analytical methods in use, impact of methods on bacterial counts and measurement uncertainty of bacterial counts. Food Microbiology, 23(1), 1-38. https://doi.org/10.1016/j.fm.2005.01.010</mixed-citation><mixed-citation xml:lang="en">Augustin, J.-C., &amp; Carlier, V. (2006). Lessons from the organization of a proficiency testing program in food microbiology by interlaboratory comparison: analytical methods in use, impact of methods on bacterial counts and measurement uncertainty of bacterial counts. Food Microbiology, 23(1), 1-38. https://doi.org/10.1016/j.fm.2005.01.010</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Boncristiani, H., Li, J. L., Evans, J. D., Pettis, J. &amp; Chen, Y. P. (2011). Scientific note on PCR inhibitors in the compound eyes of honey bees, Apis mellifera. Apidologie, 42, 457-460. https://doi.org/10.1007/s13592-011-0009-9</mixed-citation><mixed-citation xml:lang="en">Boncristiani, H., Li, J. L., Evans, J. D., Pettis, J. &amp; Chen, Y. P. (2011). Scientific note on PCR inhibitors in the compound eyes of honey bees, Apis mellifera. Apidologie, 42, 457-460. https://doi.org/10.1007/s13592-011-0009-9</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Bulgasem, B. Y., Lani, M. N., Hassan, Z., Wan Yusoff, W. M., &amp; Fnaish, S. G. (2016). Antifungal Activity of Lactic Acid Bacteria Strains Isolated From Natural Honey Against Pathogenic Candida Species. My-cobiology, 44(4), 302-309. https://doi.org/10.5941/MYCO.2016.44.4.302</mixed-citation><mixed-citation xml:lang="en">Bulgasem, B. Y., Lani, M. N., Hassan, Z., Wan Yusoff, W. M., &amp; Fnaish, S. G. (2016). Antifungal Activity of Lactic Acid Bacteria Strains Isolated From Natural Honey Against Pathogenic Candida Species. My-cobiology, 44(4), 302-309. https://doi.org/10.5941/MYCO.2016.44.4.302</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Campeau, M. E. M., &amp; Patel, R. (2014). Antibiofilm activity of manuka honey in combination with antibiotics. Hindawi Publishing Corporation International Journal of Bacteriology, 2014, 795281. https://doi.org/10.1155/2014/795281</mixed-citation><mixed-citation xml:lang="en">Campeau, M. E. M., &amp; Patel, R. (2014). Antibiofilm activity of manuka honey in combination with antibiotics. Hindawi Publishing Corporation International Journal of Bacteriology, 2014, 795281. https://doi.org/10.1155/2014/795281</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Chien, H.-Y., Shih, A.-T., Yang, B.-S., &amp; Hsiao, V. K. S. (2019). Fast honey classification using infrared spectrum and machine learning. Mathematical Biosciences and Engineering, 16(6), 6874-6891. https://doi.org/10.3934/mbe.2019344</mixed-citation><mixed-citation xml:lang="en">Chien, H.-Y., Shih, A.-T., Yang, B.-S., &amp; Hsiao, V. K. S. (2019). Fast honey classification using infrared spectrum and machine learning. Mathematical Biosciences and Engineering, 16(6), 6874-6891. https://doi.org/10.3934/mbe.2019344</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Fernandez, L. A., Ghilardi, C., Hoffmann, B., Busso, C., &amp; Gallez, L. M. (2017). Microbiological quality of honey from the pampas region (argentina) throughout the extraction process. Ryobiology, 49(1), 69-72. https://doi.org/10.1016/j.ram.2016.05.010</mixed-citation><mixed-citation xml:lang="en">Fernandez, L. A., Ghilardi, C., Hoffmann, B., Busso, C., &amp; Gallez, L. M. (2017). Microbiological quality of honey from the pampas region (argentina) throughout the extraction process. Ryobiology, 49(1), 69-72. https://doi.org/10.1016/j.ram.2016.05.010</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Gisder, S., &amp; Genersch, E. (2015). Special issue: honey bee viruses. Viruses, 7(10), 5603-5608. https://doi.org/10.3390/v7102885</mixed-citation><mixed-citation xml:lang="en">Gisder, S., &amp; Genersch, E. (2015). Special issue: honey bee viruses. Viruses, 7(10), 5603-5608. https://doi.org/10.3390/v7102885</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P., &amp; Estevinho, L. (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from portugal. Food and Chemical Toxicology, 48(2), 544-548. https://doi.org/10.1016/j.fct.2009.11.029</mixed-citation><mixed-citation xml:lang="en">Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P., &amp; Estevinho, L. (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from portugal. Food and Chemical Toxicology, 48(2), 544-548. https://doi.org/10.1016/j.fct.2009.11.029</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Hegazi, A. G., Al Guthami, F. M., Al Gethami, A. F. M., Allah, F. M. A., Saleh, A. A., Fouad, E. A. (2017) Potential antibacterial activity of some Saudi Arabia honey. Veterinary World, 10(2), 233-237. https://doi.org/10.14202/vetworld.2017.233-237</mixed-citation><mixed-citation xml:lang="en">Hegazi, A. G., Al Guthami, F. M., Al Gethami, A. F. M., Allah, F. M. A., Saleh, A. A., Fouad, E. A. (2017) Potential antibacterial activity of some Saudi Arabia honey. Veterinary World, 10(2), 233-237. https://doi.org/10.14202/vetworld.2017.233-237</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Johnston, M., McBride, M., Dahiya, D., Owusu-Apent-en, R., Nigam, P. S. (2018). Antibacterial activity of Manuka honey and its components: An overview. AIMS Microbiology, 4(4), 655-664. https://doi.org/10.3934/microbiol.2018.4.655</mixed-citation><mixed-citation xml:lang="en">Johnston, M., McBride, M., Dahiya, D., Owusu-Apent-en, R., Nigam, P. S. (2018). Antibacterial activity of Manuka honey and its components: An overview. AIMS Microbiology, 4(4), 655-664. https://doi.org/10.3934/microbiol.2018.4.655</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Kwakman, P. H. S., &amp; Zaat, S. A. J. (2012). Antibacterial components of honey. International Union of Biochemistry and Molecular Biology, 64(1), 48-55. https://doi.org/10.1002/iub.578</mixed-citation><mixed-citation xml:lang="en">Kwakman, P. H. S., &amp; Zaat, S. A. J. (2012). Antibacterial components of honey. International Union of Biochemistry and Molecular Biology, 64(1), 48-55. https://doi.org/10.1002/iub.578</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Kwong, W. K., Mancenido, A. L. &amp; Moran, N. A. (2017). Immune system stimulation by the native gut microbiota of honey bees. Royal Society Open Science, 4(2),170003. https://doi.org/10.1098/rsos.170003</mixed-citation><mixed-citation xml:lang="en">Kwong, W. K., Mancenido, A. L. &amp; Moran, N. A. (2017). Immune system stimulation by the native gut microbiota of honey bees. Royal Society Open Science, 4(2),170003. https://doi.org/10.1098/rsos.170003</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Liu, Q., Lei, J., &amp; Kadowaki, T. (2019). Gene disruption of honey bee trypanosomatid parasite, lotmaria passim, by CRISPR/Cas9 system. Frontiers in Cellular and Infection Microbiology, 9(126), 1-12. https://doi.org/10.3389/fcimb.2019.00126</mixed-citation><mixed-citation xml:lang="en">Liu, Q., Lei, J., &amp; Kadowaki, T. (2019). Gene disruption of honey bee trypanosomatid parasite, lotmaria passim, by CRISPR/Cas9 system. Frontiers in Cellular and Infection Microbiology, 9(126), 1-12. https://doi.org/10.3389/fcimb.2019.00126</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Mama, M., Teshome, T., Detamo, J. (2019). Antibacterial activity of honey against methicillin-resistant staphylococcus aureus: a laboratory-based experimental study. International Journal of Microbiology, 2019, 7686130. https://doi.org/10.1155/2019/7686130</mixed-citation><mixed-citation xml:lang="en">Mama, M., Teshome, T., Detamo, J. (2019). Antibacterial activity of honey against methicillin-resistant staphylococcus aureus: a laboratory-based experimental study. International Journal of Microbiology, 2019, 7686130. https://doi.org/10.1155/2019/7686130</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Matovic, К., Ciric, J., Kaljevic, V., Nedic, N., Jevtic, G., Vaskovic, N., &amp; Baltic , M. Z.(2019). Physicochemical Parameters and Microbiological Status of Honey Produced in an Urban Environment in Serbia. Environmental Science and Pollution Research, 25(8), 14148-14157. https://doi.org/10.1007/s11356-018-1659-1</mixed-citation><mixed-citation xml:lang="en">Matovic, К., Ciric, J., Kaljevic, V., Nedic, N., Jevtic, G., Vaskovic, N., &amp; Baltic , M. Z.(2019). Physicochemical Parameters and Microbiological Status of Honey Produced in an Urban Environment in Serbia. Environmental Science and Pollution Research, 25(8), 14148-14157. https://doi.org/10.1007/s11356-018-1659-1</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Oliveira, A., Ribeiro, H. G., Silva, A. C.,Silva, M. D., Sousa, J. C., Rodrigues, C. F.,Melo, L. D. R., Henriques, A. F., &amp; Sillankorva, S. (2017). Synergistic Antimicrobial Interaction between Honey and Phage against Escherichia coli Biofilms. Frontiers in Microbiology, 8, 664-302. https://doi.org/10.3389/fmicb.2017.02407</mixed-citation><mixed-citation xml:lang="en">Oliveira, A., Ribeiro, H. G., Silva, A. C.,Silva, M. D., Sousa, J. C., Rodrigues, C. F.,Melo, L. D. R., Henriques, A. F., &amp; Sillankorva, S. (2017). Synergistic Antimicrobial Interaction between Honey and Phage against Escherichia coli Biofilms. Frontiers in Microbiology, 8, 664-302. https://doi.org/10.3389/fmicb.2017.02407</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Pajor, M., Worobo, R., Milewski, S., &amp; Szweda, P. (2018). The antimicrobial potential of bacteria isolated from honey samples produced in the apiaries located in pomeranian voivodeship in northern poland. International Journal of Environmental Research and Public Health, 15(9), 2002. https://doi.org/10.3390/ijerph15092002</mixed-citation><mixed-citation xml:lang="en">Pajor, M., Worobo, R., Milewski, S., &amp; Szweda, P. (2018). The antimicrobial potential of bacteria isolated from honey samples produced in the apiaries located in pomeranian voivodeship in northern poland. International Journal of Environmental Research and Public Health, 15(9), 2002. https://doi.org/10.3390/ijerph15092002</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Regan, T., Barnett, M. W., Laetsch, D. R., Bush, S. J., Wragg, D., Budge, G. E., Highet, F., Dainat, B., de Miranda, J. R., Watson, M., Blaxter, M., &amp; Freeman, T. C(2018). Characterisation of the british honey bee metagenome. Nature Communications, 9(1), 1-13. https://doi.org/10.1038/s41467-018-07426-0</mixed-citation><mixed-citation xml:lang="en">Regan, T., Barnett, M. W., Laetsch, D. R., Bush, S. J., Wragg, D., Budge, G. E., Highet, F., Dainat, B., de Miranda, J. R., Watson, M., Blaxter, M., &amp; Freeman, T. C(2018). Characterisation of the british honey bee metagenome. Nature Communications, 9(1), 1-13. https://doi.org/10.1038/s41467-018-07426-0</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Ruiz-Argueso, T., &amp; Rodriguez-Navarro, A. (1975). Microbiology of Ripening Honey. Applied Microbiology, 30(6), 893-896. https://doi.org/10.1128/AEM.30.6.893-896.1975</mixed-citation><mixed-citation xml:lang="en">Ruiz-Argueso, T., &amp; Rodriguez-Navarro, A. (1975). Microbiology of Ripening Honey. Applied Microbiology, 30(6), 893-896. https://doi.org/10.1128/AEM.30.6.893-896.1975</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Sereia, M. J., Alves, E. M., Toledo, V. A. A., Marchini, L. C., Faquinello, P., Sekine, E. S., &amp; Wielewski, P. (2011). Microbial flora in organic honey samples of africanized honeybees from Parana river islands. Food Science and Technology, 31(2), 462-466. https://doi.org/10.1590/S0101-20612011000200028</mixed-citation><mixed-citation xml:lang="en">Sereia, M. J., Alves, E. M., Toledo, V. A. A., Marchini, L. C., Faquinello, P., Sekine, E. S., &amp; Wielewski, P. (2011). Microbial flora in organic honey samples of africanized honeybees from Parana river islands. Food Science and Technology, 31(2), 462-466. https://doi.org/10.1590/S0101-20612011000200028</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Sereia, M. J., Perdoncini, M. R. F. G., Mar^o, P. H., Parpi-nelli, R. S., De Lima, E. G., &amp; Anjo, F. A. (2017). Techniques for the evaluation of microbiological quality in honey. in V. de A. A. de Toledo (Ed.), Honey Analysis (pp.233-258). https://doi.org/10.5772/67086</mixed-citation><mixed-citation xml:lang="en">Sereia, M. J., Perdoncini, M. R. F. G., Mar^o, P. H., Par-pinelli, R. S., De Lima, E. G., &amp; Anjo, F. A. (2017). Techniques for the evaluation of microbiological quality in honey. in V. de A. A. de Toledo (Ed.), Honey Analysis (pp.233-258). https://doi.org/10.5772/67086</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Silva, M. S., Rabadzhiev, Y., Eller, M. R., Iliev, I., Ivanova, I., &amp; Santana, W. C. (2017). Microorganisms in Honey. In V. de A. A. de Toledo (Ed.), Honey Analysis (pp.233-258). https://doi.org/10.5772/67262</mixed-citation><mixed-citation xml:lang="en">Silva, M. S., Rabadzhiev, Y., Eller, M. R., Iliev, I., Ivanova, I., &amp; Santana, W. C. (2017). Microorganisms in Honey. In V. de A. A. de Toledo (Ed.), Honey Analysis (pp.233-258). https://doi.org/10.5772/67262</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Tudor, L., Mitranescu, E., Gali§ A. M., Ilie, L. I., &amp; Ceau^i, C. (2006). Microbiological and physicochemical analysis of honey from southern romania. Electronic Journal of Environmental, Agricultural and Food Chemistry, 9(9), 1485-1494.</mixed-citation><mixed-citation xml:lang="en">Tudor, L., Mitranescu, E., Gali§ A. M., Ilie, L. I., &amp; Ceau^i, C. (2006). Microbiological and physicochemical analysis of honey from southern romania. Electronic Journal of Environmental, Agricultural and Food Chemistry, 9(9), 1485-1494.</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Vazquez-Quinones, C. R., Moreno-Terrazas, R., Natividad-Bonifacio, I., Quinones-Ramirez, E. I., &amp; Vazquez-Salinas, C. (2017). Microbiological Assessment of Honey in Mexico. Revista Argentina de Mi-crobiologia, 50(1), 75-80. https://doi.org/10.1016/j.ram.2017.04.005</mixed-citation><mixed-citation xml:lang="en">Vazquez-Quinones, C. R., Moreno-Terrazas, R., Natividad-Bonifacio, I., Quinones-Ramirez, E. I., &amp; Vazquez-Salinas, C. (2017). Microbiological Assessment of Honey in Mexico. Revista Argentina de Mi-crobiologia, 50(1), 75-80. https://doi.org/10.1016/j.ram.2017.04.005</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Vejnovic, B., Stevanovic, J., Schwarz, R. S., Schwarz R., Aleksic, N., Mirilovic, M., Jovanovic, N. M., Stani-mirovic, Z. (2018). Quantitative pcr assessment of lotmaria passim in apis mellifera colonies co-in-fected naturally with nosema ceranae. Journal of Invertebrate Pathology, 151, 76-81. https://doi.org/10.1016/j.jip.2017.11.003</mixed-citation><mixed-citation xml:lang="en">Vejnovic, B., Stevanovic, J., Schwarz, R. S., Schwarz R., Aleksic, N., Mirilovic, M., Jovanovic, N. M., Stani-mirovic, Z. (2018). Quantitative pcr assessment of lotmaria passim in apis mellifera colonies co-in-fected naturally with nosema ceranae. Journal of Invertebrate Pathology, 151, 76-81. https://doi.org/10.1016/j.jip.2017.11.003</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
