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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">hfb</journal-id><journal-title-group><journal-title xml:lang="ru">Health, Food &amp; Biotechnology</journal-title><trans-title-group xml:lang="en"><trans-title>Health, Food &amp; Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2712-7648</issn><publisher><publisher-name>РОСБИОТЕХ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.36107/hfb.2019.i2.s245</article-id><article-id custom-type="elpub" pub-id-type="custom">hfb-17</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОТЕХНОЛОГИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BIOTECHNOLOGY</subject></subj-group></article-categories><title-group><article-title>Разработка технологии получения фитомеланинов из отходов масличного производства</article-title><trans-title-group xml:lang="en"><trans-title>Development of Technologies for Producing Phytomelanines on Waste Oil Production</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Иванова</surname><given-names>А. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Ivanova</surname><given-names>L. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Иванова Людмила Афанасьевна</p><p>125080, Москва, Волоколамское шоссе, дом 11</p></bio><bio xml:lang="en"><p>Lyudmila A. Ivanova</p><p>11 Volokolamskoe highway, Moscow, 125080</p></bio><email xlink:type="simple">biotech@mgupp.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фоменко</surname><given-names>И. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Fomenko</surname><given-names>I. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Фоменко Иван Андреевич</p><p>125080, Москва, Волоколамское шоссе, дом 11</p><p>127299, город Москва, ул. Клары Цеткин, дом 4</p></bio><bio xml:lang="en"><p>Ivan A. Fomenko</p><p>11 Volokolamskoe highway, Moscow, 125080</p></bio><email xlink:type="simple">iv.fomenko@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сергеева</surname><given-names>Д. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Sergeeva</surname><given-names>D. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сергеева Дарья Александровна</p><p>125080, Москва, Волоколамское шоссе, дом 11</p></bio><bio xml:lang="en"><p>Darya A. Sergeeva</p><p>11 Volokolamskoe highway, Moscow, 125080</p></bio><email xlink:type="simple">d.sergeeva98@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чурмасова</surname><given-names>Л. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Churmasova</surname><given-names>L. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Чурмасова Людмила Алексеевна</p><p>125080, Москва, Волоколамское шоссе, дом 11</p></bio><bio xml:lang="en"><p>Lyudmila A. Churmasova</p><p>11 Volokolamskoe highway, Moscow, 125080</p></bio><email xlink:type="simple">ludmila-churmasova@yandex.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кабаржан</surname><given-names>Ж. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Kabarzhan</surname><given-names>Zh.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кабаржан Женисбай Кенжетайулы</p><p>050010, город, Алматы, проспект Абая, дом 8</p></bio><bio xml:lang="en"><p>Zhenisbai Kabarzhan</p><p>8 Abay ave., Almaty, 050010</p></bio><email xlink:type="simple">kabarzhan.zhenisbai@mail.ru</email><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff xml:lang="en" id="aff-1"><institution>Moscow State University of Food Production</institution><country>Russian Federation</country></aff><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБОУ ВО «Московский государственный университет пищевых производств»; Институт «Прикладной биохимии и машиностроения» (ОАО «Биохиммаш»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Moscow State University of Food Production</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>ФГБОУ ВО «Московский государственный университет пищевых производств»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Moscow State University of Food Production</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Казахский национальный аграрный университет</institution><country>Казахстан</country></aff><aff xml:lang="en"><institution>Kazakh National Agrain University</institution><country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>04</day><month>05</month><year>2021</year></pub-date><volume>1</volume><issue>2</issue><fpage>136</fpage><lpage>146</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Иванова А.Л., Фоменко И.А., Сергеева Д.А., Чурмасова Л.А., Кабаржан Ж.К., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Иванова А.Л., Фоменко И.А., Сергеева Д.А., Чурмасова Л.А., Кабаржан Ж.К.</copyright-holder><copyright-holder xml:lang="en">Ivanova L.A., Fomenko I.A., Sergeeva D.A., Churmasova L.A., Kabarzhan Z.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.hfb-journal.ru/jour/article/view/17">https://www.hfb-journal.ru/jour/article/view/17</self-uri><abstract><p>Фитомеланин – уникальный компонент растительного происхождения, полученный из лузги подсолнечника, обладающий фото- и радиопротекторным действием, эффективно защищающий кожу от повреждающего воздействия УФ-лучей разной длины волны. Меланин содержится в любых живых организмах, в том числе и в растениях. Выявлено, что препараты с меланином предотвращают язвообразование, снижают число кровоизлияний в слизистой желудка и препятствует уменьшению общей массы тела в условиях стресса. Присутствие меланина в пищевых продуктах и изделиях способствует их длительному хранению. Водные экстракты, стабилизированные фитомеланином, дают возможность получить принципиально новую по своим свойствам лечебную косметику. Поиск путей получения фитомеланинов из отходов растительного сырья является актуальной задачей, стоящей перед научным сообществом. Коллективом исследователей предложен способ получения водорастворимых фитомеланинов из подсолнечной лузги, являющейся отход масличного производства. Основным этапом получения является щелочная экстракция, протекающая при температуре 120 °С в течение 1 ч. В качестве экстрагента предложено использовать раствор едкого натра различной концентрации. Полученный экстракт подкисляют раствором соляной кислоты до pH pH 1,0 – 2,0, отделяют, нейтрализуют и сушат. Для полученной субстанции подтвердили меланоидных характер с помощью качественных реакций, определили адсорбционную способность по отношению к метиленовой сини. Налучшие образцы имели активность от 50 до 79 мг/г сухого вещества. С помощью модельной тест-системы из желтка куриного яйца была определена общая антиоксидантная активность, которая составила от 10% до 28%. Полученная субстанция может быть использована в качестве лечебно-профилактического препарата или биологически активной добавки к пище.</p></abstract><trans-abstract xml:lang="en"><p>Phytomelanin is a unique [M1] component of plant origin, obtained from the husks of sunflower, having photo and radioprotective effects, effectively protecting the skin from the damaging effects of UV rays of different wavelengths. Melanin is found in all living organisms, including plants. Revealed that preparations with melanin prevent ulceration, reduce the number of hemorrhages in the stomach mucosa and prevent a decrease total body weight in stress condition. The presence of melanin in food products and products contributes to their long-term storage. Water extracts stabilized with phytomelanin gives a possibility to obtain healing cosmetics that are fundamentally new in their properties. The search for ways to obtain phytomelanins from plant waste is an actual task facing the scientific community. A collective of researchers has proposed a method for producing water-soluble phytomelanins from sunflower husk, which is an oilseed waste. The main stage of obtaining is alkaline extraction, continuing at a temperature of 120 ° C for 1 h. It is offered to use a solution of caustic soda with various concentration as an extractant. The resulting extract acidified with a solution of hydrochloric acid to a pH of 1.0 to 2.0, separated, neutralized and dried. For the obtained substance confirmed the character of melanoid using qualitative reactions, and the adsorption ability with relation to methylene blue was determined. The best exemplars had activity from 50 to 79 mg / g dry matter. Via a model test system from chicken yolk, the total antioxidant activity was determined, which ranged from 10% to 28%. The resulting substance can be used as a therapeutic drug or biologically active food supplement.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>фитомеланины</kwd><kwd>лузга подсолнечника</kwd><kwd>общая антиоксидантная активность</kwd><kwd>адсорбционная способность</kwd><kwd>стабилизатор</kwd><kwd>фотопротектор</kwd></kwd-group><kwd-group xml:lang="en"><kwd>phytomelanin</kwd><kwd>husks of sunflower</kwd><kwd>total antioxidant activity</kwd><kwd>adsorption ability</kwd><kwd>stabilizer</kwd><kwd>photoprotector</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Иванова, Г. А., Докторов, Л. Ю., Сысоева, М. А., &amp; Кутырев, Г. А. (2008). Использование азотосодержащего гиперразветвленного полимера для повышения антиоксидантной активности меланинов чаги. Химия растительного сырья, 2, 75–80.</mixed-citation><mixed-citation xml:lang="en">Ivanova, G. A., Doktorov, L. Yu., Sysoeva, M. A., &amp; Kutyrev, G. A. (2008). Using a nitrogen-containing hyperbranched polymer to increase the antioxidant activity of chaga melanins. Himiya rastitel’nogo syr’yа [Chemistry of plant raw materials], 2, 75-80.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Кузнецова, О. Ю., Шаехов, М. Ф., &amp; Зиятдинова, Г. К. (2019). Экстракты и меланины чаги, полученный после плазменной обработки сырья. Ученые записки казанского университета, 161(2), 211-221. https://doi.org/10.26907/2542-064X.2019.2.211-221</mixed-citation><mixed-citation xml:lang="en">Kuznetsova, O. Yu., Shaekhov, M. F., &amp; Ziyatdinova, G. K. (2019). Extracts and melanins of chaga obtained after plasma processing of raw materials. Uchenye zapiski kazanskogo universiteta [Scientific notes of Kazan University], 161(2), 211-221. https://doi.org/10.26907/2542-064X.2019.2.211-221</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Островский, М. А., &amp; Донцов, А. Е. (1985). Физиологические функции меланина в организме. Физиология человека, 11(4), 670-678.</mixed-citation><mixed-citation xml:lang="en">Ostrovsky, M. A., &amp; Dontsov, A. E. (1985). Physiological functions of melanin in the body. Fiziologiya cheloveka [Human physiology], 11(4), 670-678.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Севрюкова, Г. А., &amp; Кириченко, М. А. (2016). Получение меланина на основе отходов маслоэкстракционного производства. Промышленная экология и безопасность жизнедеятельности Волгоградского государственного технического университета, 1, 125.</mixed-citation><mixed-citation xml:lang="en">Sevryukova, G. A., &amp; Kirichenko, M. A. (2016). Production of melanin based on waste oil extraction production. Promyshlennaya ekologiya i bezopasnost’ zhiznedeyatel’nosti» Volgogradskogo gosudarstvennogo tekhnicheskogo universiteta [Industrial Ecology and Life Safety ”Volgograd State Technical University], 1, 125.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Юасифов, Э. Ю. (1987). Влияние меланина на свободно – радикальное состояние гамма – облученных белков и липидов. Радиобиология, 27(1), 8-11.</mixed-citation><mixed-citation xml:lang="en">Yusifov, E. Yu. (1987). Influence of melanin on the free radical state of gamma-irradiated proteins and lipids. Radiobiology [Radiobiology], 27(1), 8-11.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Adams, R., Van Bogaert, L., &amp; Ecken, H. (1994). Striatonigral degeneration. Journal of Neuropathology and Experimental Neurology, 23, 584–593.</mixed-citation><mixed-citation xml:lang="en">Adams, R., Van Bogaert, L., &amp; Ecken, H. (1994). Striatonigral degeneration. Journal of Neuropathology and Experimental Neurology, 23, 584–593.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Balandaykin, M. E., &amp; Zmitrovich, I. V. (2015). Review on chaga medicinal mushroom, Inonotus obliquus (higher basidiomycetes): Realm of medicinal applications and approaches on estimating its resource potential. International Journal of Medicinal Mushrooms, 17(2), 95–104. https://doi.org/10.1615/intjmedmushrooms.v17.i2.10</mixed-citation><mixed-citation xml:lang="en">Balandaykin, M. E., &amp; Zmitrovich, I. V. (2015). Review on chaga medicinal mushroom, Inonotus obliquus (higher basidiomycetes): Realm of medicinal applications and approaches on estimating its resource potential. International Journal of Medicinal Mushrooms, 17(2), 95–104. https://doi.org/10.1615/intjmedmushrooms.v17.i2.10</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Chung, M. J. (2010) Anticancer activity of subfractions containing pure compounds of Chaga mushroom (Inonotus obliquus) extract in human cancer cells and in Balbc/c mice bearing Sarcoma-180 cells. Nutrition research and practice, 4(3), 177–182. https://doi.org/10.4162/nrp.2010.4.3.177</mixed-citation><mixed-citation xml:lang="en">Chung, M. J. (2010) Anticancer activity of subfractions containing pure compounds of Chaga mushroom (Inonotus obliquus) extract in human cancer cells and in Balbc/c mice bearing Sarcoma-180 cells. Nutrition research and practice, 4(3), 177–182. https://doi.org/10.4162/nrp.2010.4.3.177</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">De-Paula, O. C., Marzinek, J., &amp; Oliveira, D. M. T. (2013). The role of fibres and the hypodermis in Compositae melanin secretion. Micron, 44, 312-316.</mixed-citation><mixed-citation xml:lang="en">De-Paula, O. C., Marzinek, J., &amp; Oliveira, D. M. T. (2013). The role of fibres and the hypodermis in Compositae melanin secretion. Micron, 44, 312-316.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">El-Obeid, A., Al-Harbi, S,. AL-Jomah, N., &amp; Hassib, A. (2006). Herbal melanin modulates tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) production. Phytomedicine: international journal of phytotherapy and phytopharmacology, 13(5), 324– 333.</mixed-citation><mixed-citation xml:lang="en">El-Obeid, A., Al-Harbi, S,. AL-Jomah, N., &amp; Hassib, A. (2006). Herbal melanin modulates tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) production. Phytomedicine: international journal of phytotherapy and phytopharmacology, 13(5), 324– 333.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">El-Obeid, A., Hassib, A., Pontén, F., &amp; Westermark, B. (2006). Effect of herbal melanin on IL-8: a possible role of Toll-like receptor 4 (TLR4). Biochemical and Biophysical research communications, 344(4), 1200–1206.</mixed-citation><mixed-citation xml:lang="en">El-Obeid, A., Hassib, A., Pontén, F., &amp; Westermark, B. (2006). Effect of herbal melanin on IL-8: a possible role of Toll-like receptor 4 (TLR4). Biochemical and Biophysical research communications, 344(4), 1200–1206.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Grossi, G.F., Durante, M., &amp; Gvalanella, G. (1998). Effects of melanin on high-LET radiation response of human epithelial cells. Radiation and environmental biophysics, 37, 63–67.</mixed-citation><mixed-citation xml:lang="en">Grossi, G.F., Durante, M., &amp; Gvalanella, G. (1998). Effects of melanin on high-LET radiation response of human epithelial cells. Radiation and environmental biophysics, 37, 63–67.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Hung, Y. C., Sava, V. M., Makan, S. Y., Chen, T. H. J., Hong, M. Y, &amp; Huang, G. S. (2002). Antioxidant activity of melanins derived from tea: comparison between different oxidative states. Food Chemistry, 78, 233-240.</mixed-citation><mixed-citation xml:lang="en">Hung, Y. C., Sava, V. M., Makan, S. Y., Chen, T. H. J., Hong, M. Y, &amp; Huang, G. S. (2002). Antioxidant activity of melanins derived from tea: comparison between different oxidative states. Food Chemistry, 78, 233-240.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Jana, B., &amp; Mukherjee, S. (2004). Notes on the distribution of phytomelanin layer in higher plants—a short communication. Journal of Pharmaceutical Biology, 4(3), 131–132.</mixed-citation><mixed-citation xml:lang="en">Jana, B., &amp; Mukherjee, S. (2004). Notes on the distribution of phytomelanin layer in higher plants—a short communication. Journal of Pharmaceutical Biology, 4(3), 131–132.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Kahlos, K., Kangas, L., &amp; Hiltunen, R. (1986). Antitumor activity of triterpenes in Inonotus obliquus. Planta Medica, 52, 554.</mixed-citation><mixed-citation xml:lang="en">Kahlos, K., Kangas, L., &amp; Hiltunen, R. (1986). Antitumor activity of triterpenes in Inonotus obliquus. Planta Medica, 52, 554.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Keles, Y., &amp; Özdemir, Ö. (2018). Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds. International Journal of Secondary Metabolite, 5(2), 140-148. https://doi.org/10.21448/ijsm.377470</mixed-citation><mixed-citation xml:lang="en">Keles, Y., &amp; Özdemir, Ö. (2018). Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds. International Journal of Secondary Metabolite, 5(2), 140-148. https://doi.org/10.21448/ijsm.377470</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Kunwar, A., Adhikary, B., Jayakumar, S., Barik, A., Chattopadhyay, S., Raghukumar, S., &amp; Priyadarsini K. (2012). Melanin, a promising radioprotector: Mechanisms of actions in a mice model. Toxicology and Applied Pharmacology 264(2), 202–211. https://doi.org/10.1016/j.taap.2012.08.002</mixed-citation><mixed-citation xml:lang="en">Kunwar, A., Adhikary, B., Jayakumar, S., Barik, A., Chattopadhyay, S., Raghukumar, S., &amp; Priyadarsini K. (2012). Melanin, a promising radioprotector: Mechanisms of actions in a mice model. Toxicology and Applied Pharmacology 264(2), 202–211. https://doi.org/10.1016/j.taap.2012.08.002</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Nakata, T., Yamada, T., Taji, S., Ohishi, H., Wada, S., Tokuda, H., Sakuma, K., &amp; Tanaka, R. (2007). Structure determination of inonotsuoxides A and B and in vivo anti-tumor promoting activity of inotodiol from the sclerotia of Inonotus obliquus. Bioorganic &amp; Medicinal Chemistry, 15(1), 257–264.</mixed-citation><mixed-citation xml:lang="en">Nakata, T., Yamada, T., Taji, S., Ohishi, H., Wada, S., Tokuda, H., Sakuma, K., &amp; Tanaka, R. (2007). Structure determination of inonotsuoxides A and B and in vivo anti-tumor promoting activity of inotodiol from the sclerotia of Inonotus obliquus. Bioorganic &amp; Medicinal Chemistry, 15(1), 257–264.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Oberg, F., Haseeb, A., Ahnfelt, M., Pontеn, F., Westermark, B., &amp; El-Obeid, A. (2009). Herbal melanin activates TLR 4/NF-kappa B signaling pathway. Phytomedicine: international journal of phytotherapy and phytopharmacology, 16(5), 477– 484. https://doi.org/10.1016/j.phymed.2008.10.008</mixed-citation><mixed-citation xml:lang="en">Oberg, F., Haseeb, A., Ahnfelt, M., Pontеn, F., Westermark, B., &amp; El-Obeid, A. (2009). Herbal melanin activates TLR 4/NF-kappa B signaling pathway. Phytomedicine: international journal of phytotherapy and phytopharmacology, 16(5), 477– 484. https://doi.org/10.1016/j.phymed.2008.10.008</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Park, K.I., Ishikawa, N., Morita, Y. Choi, J.D., Hoshino, A., Iida, S. (2007). A bHLH regulatory gene in the common morning glory, Ipomoea purpurea, controls anthocyanin biosynthesis in flowers, proanthocyanidin and phytomelanin pigmentation in seeds, and seed trichome formation. The Plant Journal, 49(4), 641-654.</mixed-citation><mixed-citation xml:lang="en">Park, K.I., Ishikawa, N., Morita, Y. Choi, J.D., Hoshino, A., Iida, S. (2007). A bHLH regulatory gene in the common morning glory, Ipomoea purpurea, controls anthocyanin biosynthesis in flowers, proanthocyanidin and phytomelanin pigmentation in seeds, and seed trichome formation. The Plant Journal, 49(4), 641-654.</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Pugh, N., Balachandran, P., Lata, H., Dayan, F., Joshi, V., Bedir, E., Makino, T., Moraes, R., Khan, I., &amp; Pasco, D. (2005). Melanin: dietary mucosal immune modulator from Echinacea and other botanical supplements. International Immunopharmacology. 5(4), 637–647.</mixed-citation><mixed-citation xml:lang="en">Pugh, N., Balachandran, P., Lata, H., Dayan, F., Joshi, V., Bedir, E., Makino, T., Moraes, R., Khan, I., &amp; Pasco, D. (2005). Melanin: dietary mucosal immune modulator from Echinacea and other botanical supplements. International Immunopharmacology. 5(4), 637–647.</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Schweitzer, A., Revskay,a E., Chu, P., Pazo, V., Friedman, M., Nosanchuk, J., Cahill, S., Frases, S., Casadevall, A., &amp; Dadachova, E. (2010). Melanin-covered nanoparticles for protection of bone marrow during radiation therapy of cancer. International Journal of Radiation Oncology Biology Physics 78(5), 1494–1502. https://doi.org/10.1016/j.ijrobp.2010.02.020</mixed-citation><mixed-citation xml:lang="en">Schweitzer, A., Revskay,a E., Chu, P., Pazo, V., Friedman, M., Nosanchuk, J., Cahill, S., Frases, S., Casadevall, A., &amp; Dadachova, E. (2010). Melanin-covered nanoparticles for protection of bone marrow during radiation therapy of cancer. International Journal of Radiation Oncology Biology Physics 78(5), 1494–1502. https://doi.org/10.1016/j.ijrobp.2010.02.020</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Shin, Y., Tamai Y., &amp; Terazawa M. (2000). Chemical constituents of Inonotus obliquus III. International Journal of Medicinal Mushrooms, 2(3), 201–207.</mixed-citation><mixed-citation xml:lang="en">Shin, Y., Tamai Y., &amp; Terazawa M. (2000). Chemical constituents of Inonotus obliquus III. International Journal of Medicinal Mushrooms, 2(3), 201–207.</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Shin, Y., Tamai, Y., &amp; Terazawa, M. (2001). Triterpenoids, steroids and a new sesquiterpen from Inonotus obliquus (Pers.: Fr.) Bond, et Sing. International Journal of Medicinal Mushrooms, 4(3), 250-256.</mixed-citation><mixed-citation xml:lang="en">Shin, Y., Tamai, Y., &amp; Terazawa, M. (2001). Triterpenoids, steroids and a new sesquiterpen from Inonotus obliquus (Pers.: Fr.) Bond, et Sing. International Journal of Medicinal Mushrooms, 4(3), 250-256.</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Shujing, S., Zhang, X., Sun, S., Zhang, L., Shan, S., &amp; Zhu, H. (2015). Production of natural melanin by Auricularia auricula and study on its molecular structure, Food Chemistry, 190, 801-807.</mixed-citation><mixed-citation xml:lang="en">Shujing, S., Zhang, X., Sun, S., Zhang, L., Shan, S., &amp; Zhu, H. (2015). Production of natural melanin by Auricularia auricula and study on its molecular structure, Food Chemistry, 190, 801-807.</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Zheng, W., Miao, K., Liu, Y., Zhao, Y., Zhang, M., Pan, S., &amp; Dai, Y. (2010). Chemical diversity of biologically active metabolites in the sclerotia of Inonotus obliquus and and submerged culture strategies for up-regulating their production. Applied Microbiology and Biotechnology, 87(4), 1237–1254. https://doi.org/10.1007/s00253-010-2682-4</mixed-citation><mixed-citation xml:lang="en">Zheng, W., Miao, K., Liu, Y., Zhao, Y., Zhang, M., Pan, S., &amp; Dai, Y. (2010). Chemical diversity of biologically active metabolites in the sclerotia of Inonotus obliquus and and submerged culture strategies for up-regulating their production. Applied Microbiology and Biotechnology, 87(4), 1237–1254. https://doi.org/10.1007/s00253-010-2682-4</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
