<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">hfb</journal-id><journal-title-group><journal-title xml:lang="ru">Health, Food &amp; Biotechnology</journal-title><trans-title-group xml:lang="en"><trans-title>Health, Food &amp; Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2712-7648</issn><publisher><publisher-name>РОСБИОТЕХ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.36107/hfb.2022.i2.s142</article-id><article-id custom-type="elpub" pub-id-type="custom">hfb-142</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОТЕХНОЛОГИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BIOTECHNOLOGY</subject></subj-group></article-categories><title-group><article-title>Повышение коллоидной стойкости безалкогольного пива за счет применения ферментного препарата "BREWERS CLAREX"</article-title><trans-title-group xml:lang="en"><trans-title>Increasing the Colloidal Stability of Non-alcoholic Beer Using "BREWERS CLAREX" Enzyme Preparation</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7885-1150</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Карпенко</surname><given-names>Дмитрий Валерьевич</given-names></name><name name-style="western" xml:lang="en"><surname>Karpenko</surname><given-names>Dmitry V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>профессор кафедры технологии бродильных производств и виноделия</p></bio><email xlink:type="simple">KarpenkoDV@mgupp.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9048-0225</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Каледин</surname><given-names>Иван Михайлович</given-names></name><name name-style="western" xml:lang="en"><surname>Kaledin</surname><given-names>Ivan M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>аспирант</p></bio><email xlink:type="simple">ivankaledin125424@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Московский государственный университет пищевых производств</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Moscow State University of Food Production</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>30</day><month>06</month><year>2022</year></pub-date><volume>4</volume><issue>2</issue><fpage>68</fpage><lpage>77</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Карпенко Д.В., Каледин И.М., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Карпенко Д.В., Каледин И.М.</copyright-holder><copyright-holder xml:lang="en">Karpenko D.V., Kaledin I.M.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.hfb-journal.ru/jour/article/view/142">https://www.hfb-journal.ru/jour/article/view/142</self-uri><abstract><sec><title>Введение</title><p>Введение. Коллоидная стойкость пива является одной их важных характеристик, обеспечивающих высокую конкурентоспособность напитка и интерес к нему потребителей. Однако готовое пиво является сложной системой, равновесие которой может быть нарушено под действием неоптимальных условий транспортировки и хранения, что приводит к потере исходной прозрачности. Это делает необходимым использование в производственном процессе таких режимов и технологических приемов, которые обеспечивают высокую коллоидную стойкость готового пива, в том числе, безалкогольного. Одним из таких приемов является применение ферментных препаратов протеолитического типа действия, снижающих в процессе производства пива концентрацию соединений белковой природы, способных участвовать в образовании коллоидных помутнений.</p></sec><sec><title>Цель</title><p>Цель. Целью нашего исследования являлось определение результатов применения ферментного препарата "BREWERS CLAREX", вносимого в сусло перед началом главного брожения.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Результаты применения ферментного препарата "BREWERS CLAREX", вносимого в сусло перед началом главного брожения, оценивали по содержанию «чувствительных» белков, пределу осаждения сульфатом аммония и мутности готового безалкогольного пива.</p></sec><sec><title>Результаты</title><p>Результаты. Установлена рациональная дозировка ферментного препарата, равная 2 см3/Гл сусла. Показано, что использование этого ферментного препарата позволяет существенно снизить содержание веществ белковой природы, способных инициировать формирование помутнений, а также повышает значение предела осаждения сульфатом аммония готового безалкогольного пива, однако прямой корреляции между применением  ферментного препарата и мутностью готового безалкогольного пива, полученного из разных партий солода, не установлено.</p></sec><sec><title>Выводы</title><p>Выводы. Исследованием показано, что использование ферментного препарата "BREWERS CLAREX" в рациональной дозировке обеспечило срок годности  готового безалкогольного пива не менее 180 сут.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction. The colloidal stability of beer is one of the important characteristics that ensure the high competitiveness of the drink and the interest of consumers in it. However, the finished beer is a complex system, the equilibrium of which can be disturbed under the influence of non-optimal conditions of transportation and storage, which leads to the loss of the original transparency. This makes it necessary to use in the production process such modes and technological methods that provide high colloidal stability of the finished beer, including non-alcoholic beer. One of these methods is the use of enzyme preparations of the proteolytic type of action, which reduce the concentration of protein compounds that can participate in the formation of colloidal opacities during the production of beer. </p></sec><sec><title>Purpose</title><p>Purpose. The purpose of our study was to determine the results of the application of the enzyme preparation "BREWERS CLAREX" added to the wort before the start of the main fermentation.</p></sec><sec><title>Materials and Methods</title><p>Materials and Methods. The results of the application of the enzyme preparation "BREWERS CLAREX" added to the wort before the start of the main fermentation were evaluated by the content of "sensitive" proteins in the finished non-alcoholic beer, its precipitation limit with ammonium sulfate and turbidity.</p></sec><sec><title>Results</title><p>Results. A rational dosage of the enzyme preparation was established, equal to 2 cm3/Gl of wort. It was found that the use of this enzyme preparation can significantly reduce the content of protein substances that can initiate the formation of turbidity, and also increases the precipitation limit of finished non-alcoholic beer with ammonium sulfate. However, a direct correlation between the use of the enzyme preparation and the turbidity of the finished non-alcoholic beer obtained from different batches of malt, was not determined.</p></sec><sec><title>Conclusions</title><p>Conclusions. It was found that the use of the “BREWERS CLAREX” enzyme preparation in a rational dosage ensured a shelf life of the finished non-alcoholic beer of at least 180 days</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>коллоидная стойкость безалкогольного пива</kwd><kwd>ферментный препарат "BREWERS CLAREX"</kwd><kwd>рациональная дозировка</kwd><kwd>содержание «чувствительных» белков</kwd><kwd>предел осаждения сульфатом аммония</kwd><kwd>мутность готового пива</kwd></kwd-group><kwd-group xml:lang="en"><kwd>colloidal stability of non-alcoholic beer</kwd><kwd>enzyme preparation "BREWERS CLAREX"</kwd><kwd>rational dosage</kwd><kwd>content of "sensitive" proteins</kwd><kwd>precipitation limit by ammonium sulfate</kwd><kwd>turbidity of finished beer</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Дедегкаев, А. Т., Афонин, Д. В. &amp; Меледина, Т. В. (2007). Комплексный подход к повышению коллоидной стойкости пива. Известия высших учебных заведений. Пищевая технология. 1(296), 54-56.</mixed-citation><mixed-citation xml:lang="en">Дедегкаев, А. Т., Афонин, Д. В. &amp; Меледина, Т. В. (2007). Комплексный подход к повышению коллоидной стойкости пива. Известия высших учебных заведений. Пищевая технология. 1(296), 54-56.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Меледина, Т. В., Дедегкаев, А. Т. &amp; Афонин, Д. В. (2011). Качество пива: стабильность вкуса и аромата, коллоидная стойкость. Санкт-Петербург: Издательский дом «Профессия», 220 с.</mixed-citation><mixed-citation xml:lang="en">Меледина, Т. В., Дедегкаев, А. Т. &amp; Афонин, Д. В. (2011). Качество пива: стабильность вкуса и аромата, коллоидная стойкость. Санкт-Петербург: Издательский дом «Профессия», 220 с.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Aldred, P., Kanauchi, M., &amp; Bamforth, C. W. (2021). An investigation into proteolysis in mashing. Journal of the Institute of Brewing, 127(1), 21-26. https://doi.org/10.1002/jib.635</mixed-citation><mixed-citation xml:lang="en">Aldred, P., Kanauchi, M., &amp; Bamforth, C. W. (2021). An investigation into proteolysis in mashing. Journal of the Institute of Brewing, 127(1), 21-26. https://doi.org/10.1002/jib.635</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Alves, K., Silva, B. &amp; Scheer, A. (2019). Beer aroma recovery and dealcoholisation by a two‐step pervaporation process. Journal of the Institute of Brewing, 126(1). https://doi.org/10.1002/jib.587</mixed-citation><mixed-citation xml:lang="en">Alves, K., Silva, B. &amp; Scheer, A. (2019). Beer aroma recovery and dealcoholisation by a two‐step pervaporation process. Journal of the Institute of Brewing, 126(1). https://doi.org/10.1002/jib.587</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Batchvarov, V., &amp; Kellner, V. (2002). Determination of sensitive proteins in beer by nephelometry - Submitted on behalf of the Analysis Committee of the European Brewery Convention. Monatsschrift fü Brauwissenschaft, 55(11-12), 235-236.</mixed-citation><mixed-citation xml:lang="en">Batchvarov, V., &amp; Kellner, V. (2002). Determination of sensitive proteins in beer by nephelometry - Submitted on behalf of the Analysis Committee of the European Brewery Convention. Monatsschrift fü Brauwissenschaft, 55(11-12), 235-236.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Bellut, K. &amp; Arendt, E. K. (2019). Chance and challenge: Non-saccharomyces yeasts in nonalcoholic and low alcohol beer brewing – A review. Journal of the American Society of Brewing Chemists, 77(2), 77–91. https://doi.org/10.1080/03610470.2019.1569452</mixed-citation><mixed-citation xml:lang="en">Bellut, K. &amp; Arendt, E. K. (2019). Chance and challenge: Non-saccharomyces yeasts in nonalcoholic and low alcohol beer brewing – A review. Journal of the American Society of Brewing Chemists, 77(2), 77–91. https://doi.org/10.1080/03610470.2019.1569452</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Buiatti, S., Bertoli, S. &amp; Passaghe, P. (2018). Influence of gluten-free adjuncts on beer colloidal stability. European Food Research and Technology, 244(5), 903–912. https://doi.org/10.1007/s00217-017-3010-3</mixed-citation><mixed-citation xml:lang="en">Buiatti, S., Bertoli, S. &amp; Passaghe, P. (2018). Influence of gluten-free adjuncts on beer colloidal stability. European Food Research and Technology, 244(5), 903–912. https://doi.org/10.1007/s00217-017-3010-3</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Cai, G., Li, X., Zhang, C., Zhang, M. &amp; Lu, J. (2016). Dextrin as the main turbidity components in wort produced from major malting barley cultivars of Jiangsu province in China. Journal of the Institute of Brewing, 122(3): 543-546. https://doi.org/10.1002/jib.356</mixed-citation><mixed-citation xml:lang="en">Cai, G., Li, X., Zhang, C., Zhang, M. &amp; Lu, J. (2016). Dextrin as the main turbidity components in wort produced from major malting barley cultivars of Jiangsu province in China. Journal of the Institute of Brewing, 122(3): 543-546. https://doi.org/10.1002/jib.356</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Catarino, M. &amp; Mendes, A. (2011). Non-alcoholic beer - a new industrial process. Separation and Purification Technology, 79(3), 342-351. https://doi.org/10.1016/j.seppur.2011.03.020</mixed-citation><mixed-citation xml:lang="en">Catarino, M. &amp; Mendes, A. (2011). Non-alcoholic beer - a new industrial process. Separation and Purification Technology, 79(3), 342-351. https://doi.org/10.1016/j.seppur.2011.03.020</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Carvalho, D. O. &amp; Guido, L. F. (2022). A review on the fate of phenolic compounds during malting and brewing: Technological strategies and beer styles. Food Chemistry, 372: 131093. https://doi.org/10.1016/j.foodchem.2021.131093</mixed-citation><mixed-citation xml:lang="en">Carvalho, D. O. &amp; Guido, L. F. (2022). A review on the fate of phenolic compounds during malting and brewing: Technological strategies and beer styles. Food Chemistry, 372: 131093. https://doi.org/10.1016/j.foodchem.2021.131093</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Castro, R., Díaz, A. B., Durán-Guerrero, E. &amp; Lasanta, C. (2022). Influence of different fermentation conditions on the analytical and sensory properties of craft beers: Hopping, fermentation temperature and yeast strain. Journal of Food Composition and Analysis, 106: 104278. https://doi.org/10.1016/j.jfca.2021.104278</mixed-citation><mixed-citation xml:lang="en">Castro, R., Díaz, A. B., Durán-Guerrero, E. &amp; Lasanta, C. (2022). Influence of different fermentation conditions on the analytical and sensory properties of craft beers: Hopping, fermentation temperature and yeast strain. Journal of Food Composition and Analysis, 106: 104278. https://doi.org/10.1016/j.jfca.2021.104278</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Chen, Z. (2020). Effect of thermodynamic factors on colloid stability. Journal of Physics: Conference Series, 1676: 012071. https://doi.org/10.1088/1742-6596/1676/1/012071</mixed-citation><mixed-citation xml:lang="en">Chen, Z. (2020). Effect of thermodynamic factors on colloid stability. Journal of Physics: Conference Series, 1676: 012071. https://doi.org/10.1088/1742-6596/1676/1/012071</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Cimini, A. &amp; Moresi, M. (2020). Innovative rough beer conditioning process free from diatomaceous earth and polyvinylpolypyrrolidone. Foods, 9(9): 1228. https://doi.org/10.3390/foods9091228</mixed-citation><mixed-citation xml:lang="en">Cimini, A. &amp; Moresi, M. (2020). Innovative rough beer conditioning process free from diatomaceous earth and polyvinylpolypyrrolidone. Foods, 9(9): 1228. https://doi.org/10.3390/foods9091228</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Clarex Brewers Brewing enzyme stabilization [Электронный ресурс]: – Режим доступа: https://www.dsm.com/food-specialties/en_US/products/beverage/brewers-clarex.html</mixed-citation><mixed-citation xml:lang="en">Clarex Brewers Brewing enzyme stabilization [Электронный ресурс]: – Режим доступа: https://www.dsm.com/food-specialties/en_US/products/beverage/brewers-clarex.html</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Dabija, A. (2019). Researches concerning colloidal stability's improvement of the beer using adsorption methods. Scientific Study and Research: Chemistry and Chemical Engineering, II(1-2). 71-76.</mixed-citation><mixed-citation xml:lang="en">Dabija, A. (2019). Researches concerning colloidal stability's improvement of the beer using adsorption methods. Scientific Study and Research: Chemistry and Chemical Engineering, II(1-2). 71-76.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Devolli, A., Dara, F., Stafasani, M., Shahinasi, E. &amp; Kodra, M. (2018). The influence of protein content on beer quality and colloidal stability. International Journal of Innovative Approaches in Agricultural Research, 2(4), 391-407. https://doi.org/10.29329/ijiaar.2018.174.12</mixed-citation><mixed-citation xml:lang="en">Devolli, A., Dara, F., Stafasani, M., Shahinasi, E. &amp; Kodra, M. (2018). The influence of protein content on beer quality and colloidal stability. International Journal of Innovative Approaches in Agricultural Research, 2(4), 391-407. https://doi.org/10.29329/ijiaar.2018.174.12</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Dostálek, P., Kotlikova, B., Fiala, J., Jelínek, L., Cerny, Z., Casensky, B. &amp; Mikulka, J. (2011). Stabilizers for increased colloidal stability of beer. Kvasny Prumysl, 57(7-8), 290-295. https://doi.org/10.18832/kp2011034</mixed-citation><mixed-citation xml:lang="en">Dostálek, P., Kotlikova, B., Fiala, J., Jelínek, L., Cerny, Z., Casensky, B. &amp; Mikulka, J. (2011). Stabilizers for increased colloidal stability of beer. Kvasny Prumysl, 57(7-8), 290-295. https://doi.org/10.18832/kp2011034</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Gribkova, I. N., Eliseev, M. N., Belkin Yu. D., Zakharov, M. A. &amp; Kosareva, O. A. (2022). The influence of biomolecule composition on colloidal beer structure. Biomolecules, 12(1), 24. https://doi.org/10.3390/biom12010024</mixed-citation><mixed-citation xml:lang="en">Gribkova, I. N.,  Eliseev, M. N., Belkin Yu. D., Zakharov, M. A. &amp; Kosareva, O. A. (2022). The influence of biomolecule composition on colloidal beer structure. Biomolecules, 12(1), 24. https://doi.org/10.3390/biom12010024</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Habschied, K., Krstanović, V., Lukinac, J., Jukić, M., Vulin, Z. &amp; Mastanjević, K. (2018). Beer – the importance of colloidal stability (non-biological haze). Fermentation. 4(4). 91. https://doi.org/10.3390/fermentation4040091</mixed-citation><mixed-citation xml:lang="en">Habschied, K., Krstanović, V., Lukinac, J., Jukić, M., Vulin, Z. &amp; Mastanjević, K. (2018). Beer – the importance of colloidal stability (non-biological haze). Fermentation. 4(4). 91. https://doi.org/10.3390/fermentation4040091</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Kaledin, I. M, &amp; and Karpenko D. V. (2022). Improving the storage stability of non-alcoholic beer. IOP Conf. Series: Earth and Environmental Science. 1052: 012106. https://doi.org/doi:10.1088/1755-1315/1052/1/012106</mixed-citation><mixed-citation xml:lang="en">Kaledin, I. M, &amp; and Karpenko D. V. (2022). Improving the storage stability of non-alcoholic beer. IOP Conf. Series: Earth and Environmental Science. 1052: 012106. https://doi.org/doi:10.1088/1755-1315/1052/1/012106</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Kristina, M., Vinko, K., Krešimir, M. &amp; Bojan, Š. (2018). Malting and brewing industries encounter Fusarium spp. related problems. Fermentation. 4(1), 3. https://doi.org/10.3390/fermentation4010003</mixed-citation><mixed-citation xml:lang="en">Kristina, M., Vinko, K., Krešimir, M. &amp; Bojan, Š. (2018). Malting and brewing industries encounter Fusarium spp. related problems. Fermentation. 4(1), 3. https://doi.org/10.3390/fermentation4010003</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Kwon, S., Orsuwan, A., Bumbudsanpharoke, N., Yoon, C., Choi, J. &amp; Ko, S. (2018). A short review of light barrier materials for food and beverage packaging. Korean Journal of Packaging Science and Technology, 24(3), 141-148. https://doi.org/10.20909/kopast.2018.24.3.141</mixed-citation><mixed-citation xml:lang="en">Kwon, S., Orsuwan, A., Bumbudsanpharoke, N., Yoon, C., Choi, J. &amp; Ko, S. (2018). A short review of light barrier materials for food and beverage packaging. Korean Journal of Packaging Science and Technology, 24(3), 141-148. https://doi.org/10.20909/kopast.2018.24.3.141</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Leiper, K., Stewart, G., Mckeown, I., Nock, T. &amp; Thompson, M. (2005). Optimising Beer Stabilisation by the Selective Removal of Tannoids and Sensitive Proteins. Journal of the Institute of Brewing, 111(2), 118-127. https://doi.org/10.1002/j.2050-0416.2005.tb00657.x</mixed-citation><mixed-citation xml:lang="en">Leiper, K., Stewart, G., Mckeown, I., Nock, T. &amp; Thompson, M. (2005). Optimising Beer Stabilisation by the Selective Removal of Tannoids and Sensitive Proteins. Journal of the Institute of Brewing, 111(2), 118-127. https://doi.org/10.1002/j.2050-0416.2005.tb00657.x</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Lingzhen, Y., Yuqing, H., Fei, D., Huajiang, N., Chengdao, L., Meixue, Z. &amp; Guoping, Z. (2015). Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L). BMC Genomics, 16(1): 449. https://doi.org/10.1186/s12864-015-1683-1</mixed-citation><mixed-citation xml:lang="en">Lingzhen, Y., Yuqing, H., Fei, D., Huajiang, N., Chengdao, L., Meixue, Z. &amp; Guoping, Z. (2015). Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L). BMC Genomics, 16(1): 449. https://doi.org/10.1186/s12864-015-1683-1</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Lopez, M. &amp; Edens, L. (2005). Effective prevention of chill-haze in beer using an acid proline-specific endoprotease from Aspergillus niger. Journal of Agricultural and Food Chemistry, 53(20), 7944–7949. https://doi.org/10.1021/jf0506535</mixed-citation><mixed-citation xml:lang="en">Lopez, M. &amp; Edens, L. (2005). Effective prevention of chill-haze in beer using an acid proline-specific endoprotease from Aspergillus niger. Journal of Agricultural and Food Chemistry, 53(20), 7944–7949. https://doi.org/10.1021/jf0506535</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Lu, Y., Bergenståhl, B. &amp; Nilsson, L. (2020). Interfacial properties and interaction between beer wort protein fractions and iso-humulone. Food Hydrocolloids, 103: 105648. https://doi.org/10.1016/j.foodhyd.2020.105648</mixed-citation><mixed-citation xml:lang="en">Lu, Y., Bergenståhl, B. &amp; Nilsson, L. (2020). Interfacial properties and interaction between beer wort protein fractions and iso-humulone. Food Hydrocolloids, 103: 105648. https://doi.org/10.1016/j.foodhyd.2020.105648</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Nehra, M., Grover, N. &amp; Gahlawat, S.. (2022). Non alcoholic beers: review and methods. Madridge Journal of Food Technology. 7(1): 1000130.</mixed-citation><mixed-citation xml:lang="en">Nehra, M., Grover, N. &amp; Gahlawat, S.. (2022). Non alcoholic beers: review and methods. Madridge Journal of Food Technology. 7(1): 1000130.</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Perretti, G., Floridi, S., Turchetti, B., Marconi, O., &amp; Fantozzi, P. (2011) Quality control of malt: turbidity problems of standard worts given by the presence of microbial cells. Journal of the Institute of Brewing, 117(2), 212– 216. https://doi.org/10.1002/j.2050-0416.2011.tb00463.x</mixed-citation><mixed-citation xml:lang="en">Perretti, G., Floridi, S., Turchetti, B., Marconi, O., &amp; Fantozzi, P. (2011) Quality control of malt: turbidity problems of standard worts given by the presence of microbial cells. Journal of the Institute of Brewing, 117(2), 212– 216. https://doi.org/10.1002/j.2050-0416.2011.tb00463.x</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Salanță, L. C., Coldea, T. E., Ignat, M. V., Pop, C. R., Tofană, M., Mudura, E., Borșa, A., Pasqualone, A. &amp; Zhao, H. (2020). Non-Alcoholic and Craft Beer Production and Challenges. Processes, 8(11): 1382. https://doi.org/10.3390/pr8111382</mixed-citation><mixed-citation xml:lang="en">Salanță, L. C., Coldea, T. E., Ignat, M. V., Pop, C. R., Tofană, M., Mudura, E., Borșa, A., Pasqualone, A. &amp; Zhao, H.  (2020). Non-Alcoholic and Craft Beer Production and Challenges. Processes, 8(11): 1382. https://doi.org/10.3390/pr8111382</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Siebert, K. (2010). The effect of beer pH on colloidal stability and stabilization - A review and recent findings. Technical Quarterly. 47(2), 1-5. https://doi.org/doi:10.1094/TQ-47-2-0607-01</mixed-citation><mixed-citation xml:lang="en">Siebert, K. (2010). The effect of beer pH on colloidal stability and stabilization - A review and recent findings. Technical Quarterly. 47(2), 1-5. https://doi.org/doi:10.1094/TQ-47-2-0607-01</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Siebert, K. J., &amp; Lynn, P. Y. (2007). The effect of beer pH on colloidal stabilization with adsorbents. Journal of the American Society of Brewing Chemists, 65(1), 52–58. https://doi.org/10.1094/ASBCJ-2007-0115-01</mixed-citation><mixed-citation xml:lang="en">Siebert, K. J., &amp; Lynn, P. Y. (2007). The effect of beer pH on colloidal stabilization with adsorbents. Journal of the American Society of Brewing Chemists, 65(1), 52–58. https://doi.org/10.1094/ASBCJ-2007-0115-01</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Sohrabvandi, S., Mousavi, S. M., Razavi, S. H., Mortazavian, A. M. &amp; Rezaei, K. (2010). Alcohol-free beer: Methods of production, sensorial defects, and healthful effects. Food Reviews International, 26(4), 335–352. https://doi.org/10.1080/87559129.2010.496022</mixed-citation><mixed-citation xml:lang="en">Sohrabvandi, S., Mousavi, S. M., Razavi, S. H., Mortazavian, A. M. &amp; Rezaei, K. (2010). Alcohol-free beer: Methods of production, sensorial defects, and healthful effects. Food Reviews International, 26(4), 335–352. https://doi.org/10.1080/87559129.2010.496022</mixed-citation></citation-alternatives></ref><ref id="cit33"><label>33</label><citation-alternatives><mixed-citation xml:lang="ru">Steiner, E., Gastl, M. &amp; Becker, T. (2011). Protein changes during malting and brewing with focus on haze and foam formation: a review. European Food Research and Technology, 232(2), 191– 204. https://doi.org/10.1007/s00217-010-1412-6</mixed-citation><mixed-citation xml:lang="en">Steiner, E., Gastl, M. &amp; Becker, T. (2011). Protein changes during malting and brewing with focus on haze and foam formation: a review. European Food Research and Technology, 232(2), 191– 204. https://doi.org/10.1007/s00217-010-1412-6</mixed-citation></citation-alternatives></ref><ref id="cit34"><label>34</label><citation-alternatives><mixed-citation xml:lang="ru">Wannenmacher, J., Gastl, M. &amp; Becker, T. (2018). Phenolic substances in beer: Structural diversity, reactive potential and relevance for brewing process and beer quality. Comprehensive Reviews in Food Science and Food Safety, 17(4), 953–988. https://doi.org.10.1111/1541-4337.12352</mixed-citation><mixed-citation xml:lang="en">Wannenmacher, J., Gastl, M. &amp; Becker, T. (2018). Phenolic substances in beer: Structural diversity, reactive potential and relevance for brewing process and beer quality. Comprehensive Reviews in Food Science and Food Safety, 17(4), 953–988. https://doi.org.10.1111/1541-4337.12352</mixed-citation></citation-alternatives></ref><ref id="cit35"><label>35</label><citation-alternatives><mixed-citation xml:lang="ru">Zheng, Y., Du, J. &amp; Li, M. (2020). Haze-active protein and turbidity in commercial barley and wheat beers at different storage temperatures. International Food Research Journal, 27(2), 295–307.</mixed-citation><mixed-citation xml:lang="en">Zheng, Y., Du, J. &amp; Li, M. (2020). Haze-active protein and turbidity in commercial barley and wheat beers at different storage temperatures. International Food Research Journal, 27(2), 295–307.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
